Human allogeneic keratinocytes cultured on acellular xenodermis: the use in healing of burns and other skin defects.
Human allogeneic keratinocytes cultured on acellular xenodermis: the use in healing of burns and other skin defects.
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发表时间：
2006
影响因子：
1
通讯作者：
E. Matoušková;L. Brož;V. Stolbová;L. Klein;R. Konigová;P. Veselý
中科院分区：
文献类型：
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作者：
E. Matoušková;L. Brož;V. Stolbová;L. Klein;R. Konigová;P. Veselý
组织工程皮肤应包括真皮层和表皮层。我们将培养的人同种异体角质形成细胞与从猪异种移植物制备的脱细胞异种真皮结合。所得到的复合皮肤被称为重组人/猪皮肤（RHPS），并且可以以未分化和分化表型培养。将未分化的RHPS浸没生长并形成1-2层角质形成细胞。分化表型（D-RHPS）生长在气液界面，形成5-20层类似于正常表皮的细胞层，包括颗粒层和角质层。未分化的RHPS具有皮肤样的稠度，并且已经成功地用于使用“倒置”施用来治疗烧伤和皮肤缺损。供皮区和深皮烧伤创面切痂或深磨皮术移植RHPS后1周左右愈合。不含角质形成细胞的简单脱细胞异种真皮也可用作供皮区、小到中等腿部溃疡和其他皮肤缺损的临时覆盖物。异皮属植物可完全灭菌并在室温下储存。
The tissue engineered skin should be composed of both dermal and epidermal layers. We combined cultured human allogeneic keratinocytes with acellular xenodermis prepared from pig xenografts. The resulting composite skin was termed recombined human/pig skin (RHPS), and could be cultured in both, undifferentiated and differentiated phenotype. The undifferentiated RHPS was grown submerged and formed 1-2 layers of keratinocytes. The differentiated phenotype (D-RHPS) was grown at the air-liquid interface and formed 5-20 cell layers similar to the normal epidermis, including the granular and horny layers. Undifferentiated RHPS has skin-like consistency and has been successfully used for treatment of burns and skin defects using "upside-down" application. Donor sites and deep dermal burn wounds prepared by tangential excision or deep dermabrasion grafted with RHPS healed in the course of about one week after keratinocyte transplantation. Simple acellular xenodermis without keratinocytes can also be used as temporary cover for donor sites, small to medium leg ulcers and other skin defects. Xenodermis can be fully sterilized and stored at the room temperature.