Vector competence of Aedes vexans (Meigen), Culex poicilipes (Theobald) and Cx. quinquefasciatus Say from Senegal for West and East African lineages of Rift Valley fever virus.
Vector competence of Aedes vexans (Meigen), Culex poicilipes (Theobald) and Cx. quinquefasciatus Say from Senegal for West and East African lineages of Rift Valley fever virus.
复制标题
DOI：
10.1186/s13071-016-1383-y
复制
发表时间：
2016-02-20
影响因子：
3.2
通讯作者：
Diallo M
中科院分区：
文献类型：
--
作者：
Ndiaye el H;Fall G;Gaye A;Bob NS;Talla C;Diagne CT;Diallo D;B A Y;Dia I;Kohl A;Sall AA;Diallo M
裂谷热病毒(RVFV；蛇毒，布尼亚病毒科)是一种由蚊子传播的人畜共患病病原体。在塞内加尔，1974年首次从伊蚊(Theobald)分离到RVFV，此后又从Ae分离到RVFV。Ae.Fowleri(De Charmoy)Ochraceus Theobald，Ae.Vexans(Meigen)、Policilipes(Theobald)、Mansonia africana(Theobald)和Ma.制服(西奥巴尔德)。然而，这些当地物种的媒介能力从未得到证实，这使得根据血清学数据和蚊子分离株提出的西非传播周期成为假设。白纹伊蚊和Cx。Poicilipes是塞内加尔最常与RVFV有关的两种常见蚊子物种，以及Cx。用西非和东非/中非血统的三个RVFV毒株进行口服喂养后，对最常见的家养物种Ququiefasciatus进行了评估。完全充血的蚊子(420 Ae.Vexans，563Cx。Ququiefasciatus和380 Cx.Poicilipes)保持在27 ± 1°C和70-80%相对湿度。分别在暴露后5、10、15和20天(DPE)用实时RT-PCR检测唾液、腿/翅和身体的RVFV基因组，以估计感染率、传播率和传播率。对所用的3个菌株进行了基因分型，以确定不同传播模式的潜在因素。Ae感染率在30.0~85.0%之间。Vexans，CX 3.3-27%。CX为8.3-46.7%。Poicilipes，Ae的传播率在10.5-370%之间。Vexans，CX为9.5-28.6%。CX为3.0-40.9%。波波利普。然而，只有东非血统被传播，传播率在Ae的13.3-33.3%之间变化。Vexans，CX占50%。Ququiefasciatus和CX的11.1%。波波利普。与Ae相比，库蚊对感染的敏感性更低。愤怒的人。与其他毒株相比，东非RVFV人源性株SH172805株NSS M节段蛋白的氨基酸变异可能解释了传播潜力的差异。我们的研究结果表明，所有受试物种都能胜任RVFV，其中Ae的作用更为重要。与库蚊相比，Vexans是东非血统中传播潜力最高的物种。本文的在线版本(doi：10.1186/s13071-0161383-y)包含补充材料，授权用户可以使用。
Rift Valley fever virus (RVFV; Phlebovirus, Bunyaviridae) is a mosquito–borne, zoonotic pathogen. In Senegal, RVFV was first isolated in 1974 from Aedes dalzieli (Theobald) and thereafter from Ae. fowleri (de Charmoy), Ae. ochraceus Theobald, Ae. vexans (Meigen), Culex poicilipes (Theobald), Mansonia africana (Theobald) and Ma. uniformis (Theobald). However, the vector competence of these local species has never been demonstrated making hypothetical the transmission cycle proposed for West Africa based on serological data and mosquito isolates. Aedes vexans and Cx. poicilipes, two common mosquito species most frequently associated with RVFV in Senegal, and Cx. quinquefasciatus, the most common domestic species, were assessed after oral feeding with three RVFV strains of the West and East/central African lineages. Fully engorged mosquitoes (420 Ae. vexans, 563 Cx. quinquefasciatus and 380 Cx. poicilipes) were maintained at 27 ± 1 °C and 70–80 % relative humidity. The saliva, legs/wings and bodies were tested individually for the RVFV genome using real-time RT-PCR at 5, 10, 15 and 20 days post exposure (dpe) to estimate the infection, dissemination, and transmission rates. Genotypic characterisation of the 3 strains used were performed to identify factors underlying the different patterns of transmission. The infection rates varied between 30.0–85.0 % for Ae. vexans, 3.3–27 % for Cx. quinquefasciatus and 8.3–46.7 % for Cx. poicilipes, and the dissemination rates varied between 10.5–37 % for Ae. vexans, 9.5–28.6 % for Cx. quinquefasciatus and 3.0–40.9 % for Cx. poicilipes. However only the East African lineage was transmitted, with transmission rates varying between 13.3–33.3 % in Ae. vexans, 50 % in Cx. quinquefasciatus and 11.1 % in Cx. poicilipes. Culex mosquitoes were less susceptible to infection than Ae. vexans. Compared to other strains, amino acid variation in the NSs M segment proteins of the East African RVFV lineage human-derived strain SH172805, might explain the differences in transmission potential. Our findings revealed that all the species tested were competent for RVFV with a significant more important role of Ae. vexans compared to Culex species and a highest potential of the East African lineage to be transmitted. The online version of this article (doi:10.1186/s13071-016-1383-y) contains supplementary material, which is available to authorized users.