Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks
Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks
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DOI：
10.1016/s1534-5807(02)00321-0
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发表时间：
2002-11-01
影响因子：
11.8
通讯作者：
Ang, SL
中科院分区：
文献类型：
--
作者：
Perea-Gomez, A;Vella, FDJ;Ang, SL
前内脏内胚层在建立小鼠胚胎的前-后极性中起着关键作用，但所需信号的分子性质仍有待确定。在这里，我们证明，地狱犬样（-/-）; Lefty 1（-/-）复合突变体可以开发一个原始的条纹异位在胚胎中。这种缺陷在含有野生型胚胎和Cerberus样-/- ; Lefty 1（-/-）胚外细胞的嵌合体中不能得到挽救，但在去除一个Nodal基因拷贝后，在Cerberus样（-/-）; Lefty 1（-/-）胚胎中得到挽救。我们的研究结果提供了一个模型，即地狱犬样和Lefty 1在前内脏内胚层限制原条形成的后端小鼠胚胎通过拮抗Nodal信号。这两种拮抗剂也需要适当的模式的原始条纹。
The anterior visceral endoderm plays a pivotal role in establishing anterior-posterior polarity of the mouse embryo, but the molecular nature of the signals required remains to be determined. Here, we demonstrate that Cerberus-like(-/-) ;Lefty1(-/-) compound mutants can develop a primitive streak ectopically in the embryo. This defect is not rescued in chimeras containing wild-type embryonic, and Cerberus-like-/- ;Lefty1(-/-) extraembryonic, cells but is rescued in Cerberus-like(-/-); Lefty1(-/-) embryos after removal of one copy of the Nodal gene. Our findings provide support for a model whereby Cerberus-like and Lefty1 in the anterior visceral endoderm restrict primitive streak formation to the posterior end of mouse embryos by antagonizing Nodal signaling. Both antagonists are also required for proper patterning of the primitive streak.