FUNCTIONAL AND STRUCTURAL-ANALYSIS OF PHOTOSYSTEM-II CORE COMPLEXES FROM SPINACH WITH HIGH OXYGEN EVOLUTION CAPACITY
FUNCTIONAL AND STRUCTURAL-ANALYSIS OF PHOTOSYSTEM-II CORE COMPLEXES FROM SPINACH WITH HIGH OXYGEN EVOLUTION CAPACITY
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DOI：
10.1111/j.1432-1033.1990.tb15458.x
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发表时间：
1990-04-20
期刊：
影响因子：
--
通讯作者：
RENGER, G
中科院分区：
文献类型：
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作者：
HAAG, E;IRRGANG, KD;RENGER, G
放氧光系统II核心复合物通过用十二烷基-β-葡聚糖溶解光系统II膜片段从菠菜制备。D-麦芽糖苷核心复合物由内源性47-kDa、43-kDa、D1和D2多肽、细胞色素b559的两个亚基和外源性33-kDa蛋白组成。在50 mM CaCl 2存在下，它们表现出1.3 ± 1.5的高氧释放速率。0.2 mmol O2. cntdot. mg叶绿素-1.cntdot. h ~（-1）与2，6-二氯苯醌或K_3 [Fe（CN）_6]为受体。这些配合物的电子显微照片显示，从顶部观察时，在一个钝角三角形结构，测量15.3 nm的一侧和10.6 nm的其他两侧。从侧视位置确定平均高度为7.3 nm。这些数据与先前报道的光系统II核心复合物的尺寸非常一致[Irrgang，K. D、Boekema，E. J.，Vater，J.和Renger，G.（1988）Eur. 178，209-217]。与以前的报告相比，外源性33-kDa亚基可以解决的第一次。当从侧面观察复合物时，它表现为小突起，并且似乎覆盖核心复合物的内腔侧，表现为厚度为1.5-3.3 nm的圆盘。
Oxygen-evolving photosystem II core complexes were prepared from spinach by solubilizing photosystem II membrane fragments with dodecyl-.beta.-D-maltoside. The core complexes consist of the intrinsic 47-kDa, 43-kDa, D1 and D2 polypeptides, the two subunits of cytochrome b559 and the extrinsic 33-kDa protein. In the presence of 50 mM CaCl2 they exhibit a high oxygen evolution rate of 1.3 .+-. 0.2 mmol O2 .cntdot. mg chlorophyll-1 .cntdot. h-1 with either 2,6-dichloro-benzoquinone or K3[Fe(CN)6] as acceptor. Electron micrographs of these complexes reveal an obtuse triangular structure in when viewed from the top, measuring 15.3 nm on one side and 10.6 nm on the other two sides. An average height of 7.3 nm was determined from the side view position. These data are in good agreement with previously reported dimensions for photosystem II core complexes [Irrgang, K.-D., Boekema, E. J., Vater, J. and Renger, G. (1988) Eur. J. Biochem. 178, 209-217]. In contrast to previous reports the extrinsic 33-kDa subunit could be resolved for the first time. It appears as a small protrusion when the complex is viewed from the side and seems to cover the lumenal side of the core complex appearing as a disk with a thickness of 1.5-3.3 nm.