Apatite Formation and Biocompatibility of a Low Young's Modulus Ti-Nb-Sn Alloy Treated with Anodic Oxidation and Hot Water.
Apatite Formation and Biocompatibility of a Low Young's Modulus Ti-Nb-Sn Alloy Treated with Anodic Oxidation and Hot Water.
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DOI：
10.1371/journal.pone.0150081
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Itoi E
中科院分区：
文献类型：
--
作者：
Tanaka H;Mori Y;Noro A;Kogure A;Kamimura M;Yamada N;Hanada S;Masahashi N;Itoi E
Ti-6Al-4V合金因其良好的耐腐蚀性和生物相容性而广泛应用于骨科植入物。然而，金属假体与人类皮质骨之间的杨氏模量差异有时会引起临床问题，由于应力屏蔽导致大腿疼痛和骨萎缩。为解决应力不平衡问题，设计了低杨氏模量的Ti-Nb-Sn合金。在这项研究中，我们评估了阳极氧化加热水处理或不加热水处理对Ti-Nb-Sn合金骨结合特性的影响。我们通过扫描电镜，XPS和x射线衍射分析检查了表面分析和磷灰石形成。我们还通过拉出试验测量失效载荷和定量组织形态学分析来评估实验动物模型的生物相容性。在Hank’s溶液中培养后，经阳极氧化和热水处理的Ti-Nb-Sn合金表面形成了丰富的磷灰石。通过XRD分析，在表面发现了强烈的磷灰石形成峰。XPS分析显示，O - 1s XPS中H2O组分增加。拔拔试验结果表明，经阳极氧化和热水处理的Ti-Nb-Sn合金棒的失效载荷大于未处理的棒。定量组织形态学分析表明，阳极氧化和热水处理诱导了杆周围较高的新骨形成。研究结果表明，经阳极氧化和热水处理的Ti-Nb-Sn合金具有更强的磷灰石形成能力、更强的骨结合能力和更高的生物相容性。Ti-Nb-Sn合金经阳极氧化和热水处理后，具有较高的成骨性和较低的应力失调，是一种很有前途的骨科植入材料。
Ti-6Al-4V alloy is widely prevalent as a material for orthopaedic implants because of its good corrosion resistance and biocompatibility. However, the discrepancy in Young’s modulus between metal prosthesis and human cortical bone sometimes induces clinical problems, thigh pain and bone atrophy due to stress shielding. We designed a Ti-Nb-Sn alloy with a low Young’s modulus to address problems of stress disproportion. In this study, we assessed effects of anodic oxidation with or without hot water treatment on the bone-bonding characteristics of a Ti-Nb-Sn alloy. We examined surface analyses and apatite formation by SEM micrographs, XPS and XRD analyses. We also evaluated biocompatibility in experimental animal models by measuring failure loads with a pull-out test and by quantitative histomorphometric analyses. By SEM, abundant apatite formation was observed on the surface of Ti-Nb-Sn alloy discs treated with anodic oxidation and hot water after incubation in Hank’s solution. A strong peak of apatite formation was detected on the surface using XRD analyses. XPS analysis revealed an increase of the H2O fraction in O 1s XPS. Results of the pull-out test showed that the failure loads of Ti-Nb-Sn alloy rods treated with anodic oxidation and hot water was greater than those of untreated rods. Quantitative histomorphometric analyses indicated that anodic oxidation and hot water treatment induced higher new bone formation around the rods. Our findings indicate that Ti-Nb-Sn alloy treated with anodic oxidation and hot water showed greater capacity for apatite formation, stronger bone bonding and higher biocompatibility for osteosynthesis. Ti-Nb-Sn alloy treated with anodic oxidation and hot water treatment is a promising material for orthopaedic implants enabling higher osteosynthesis and lower stress disproportion.