Recent advances in engineering microbial rhodopsins for optogenetics.
Recent advances in engineering microbial rhodopsins for optogenetics.
复制标题
用于光遗传学的工程微生物动蛋白的最新进展。
DOI：
10.1016/j.sbi.2015.05.001
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发表时间：
2015-08
影响因子：
6.8
通讯作者：
Arnold FH
中科院分区：
文献类型：
--
作者：
McIsaac RS;Bedbrook CN;Arnold FH
微生物视紫红质的蛋白质工程已经成功地产生了具有改进性质的变体，用于光遗传学。该膜蛋白家族的成员可以充当神经元活动的致动器和传感器。嵌合、结构引导突变和定向进化已被证明是调谐吸收波长、改变离子特异性和增加荧光的有效策略。这些方法促进了有用的光遗传学工具的开发，并且在某些情况下，已经产生了对视紫红质结构-功能关系的见解。
Protein engineering of microbial rhodopsins has been successful in generating variants with improved properties for applications in optogenetics. Members of this membrane protein family can act as both actuators and sensors of neuronal activity. Chimeragenesis, structure-guided mutagenesis, and directed evolution have proven effective strategies for tuning absorption wavelength, altering ion specificity and increasing fluorescence. These approaches facilitate the development of useful optogenetic tools and, in some cases, have yielded insights into rhodopsin structure-function relationships.