Regulation of conceptus adhesion by endometrial CXC chemokines during the implantation period in sheep
Regulation of conceptus adhesion by endometrial CXC chemokines during the implantation period in sheep
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DOI：
10.1002/mrd.20496
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发表时间：
2006-07-01
影响因子：
2.5
通讯作者：
Christenson, Ronald K.
中科院分区：
文献类型：
--
作者：
Imakawa, Kazuhiko;Imai, Misa;Christenson, Ronald K.
为了更好地了解反刍有蹄类动物孕体粘附于母体子宫内膜的生化机制，本研究旨在阐明趋化因子和细胞外基质（ECM）成分在绵羊囊胚粘附于子宫内膜的调控中的作用。除了趋化因子干扰素-γ诱导蛋白10 kDa（IP-10，CXCL 10）外，趋化因子受体CXCR 3还识别两种其他趋化因子; IFN-γ诱导的单核因子（IFN-γ，CXCL 9）和IFN-诱导T细胞a化学引诱物（I-TAC，CXCL 11）。与CXCL 10类似，CXCL 9和CXCL 11在围着床期子宫中表达，并且通过添加IFN-tau或IFN-γ刺激来自第14天周期母羊的子宫内膜外植体中的CXCL 9 mRNA表达。在没有ECM成分的情况下，孕体细胞粘附在妊娠第14天较低，在第17天增加2.5倍;第20天的粘附是第14天的1/10。在检查的各种ECM成分中，使用纤连蛋白时滋养层粘附最大。虽然第14天的孕体没有表现出很大的粘附活性纤连蛋白，第17天的滋养层，和第20天的绒毛膜表现出2.3倍和50倍的增加，分别与CXCL 9或CXCL 10的治疗增强。这些结果表明，通过子宫内膜纤连蛋白和趋化因子，绵羊孕体细胞获得的能力，附着在子宫内膜在植入前的时期，但在此期间，孕体获得粘附能力的分子机制有待进一步研究。
To gain a better understanding of biochemical mechanisms of conceptus adhesion to the maternal endometrium in ruminant ungulates, the present study was performed to clarify roles of chemokines and extracellular matrix (ECM) components in the regulation of ovine blastocyst attachment to the endometrium. In addition to the chemokine, interferon-gamma inducible protein 10 kDa (IP-10, CXCL10), the chemokine receptor, CXCR3, also recognizes two other chemokines; monokine induced by IFN-gamma (MIG, CXCL9) and IFN-inducible T cell a chemoattractant (I-TAC, CXCL11). Similar to CXCL10, CXCL9, and CXCL11 were expressed in the uterus during the peri-implantation period, and CXCL9 mRNA expression was stimulated in endometrial explants from day 14 cyclic ewes by the addition of IFN-tau or IFN-gamma. Without ECM components, conceptus cell adhesion was low on day 14 of gestation and exhibited a 2.5-fold increase on day 17; adhesiveness on day 20 was 1/10 of that on day 14. Among various ECM components examined, trophoblast adhesion was greatest when fibronectin was used. Although day 14 conceptuses did not show much adhesive activity to fibronectin, day 17 trophoblast, and day 20 chorionic membrane exhibited 2.3-fold and 50-fold increase, respectively, which was enhanced by treatment with CXCL9 or CXCL10. These results indicate that through endometrial fibronectin and chemokines, ovine conceptus cells gain the ability to attach to the endometrium during pre-implantation period; however, elucidation of molecular mechanisms by which the conceptus acquires the adhesive ability during this time period awaits further investigation.