Dynamic light scattering microscopy. A novel optical technique to image submicroscopic motions. I: theory.
Dynamic light scattering microscopy. A novel optical technique to image submicroscopic motions. I: theory.
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DOI：
10.1529/biophysj.103.033837
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发表时间：
2004-08
影响因子：
3.4
通讯作者：
R. Dzakpasu;D. Axelrod
中科院分区：
文献类型：
--
作者：
R. Dzakpasu;D. Axelrod
光学显微镜技术的理论基础，图像动态散射光波动衰减率（动态光散射显微镜）的发展。它示出，散射中心之间的相对运动，甚至小于显微镜的光学分辨率足以产生显着的相位变化，导致在图像平面中的干涉强度波动。这些强度波动的时间自相关函数的时间尺度和时间依赖性。空间相关距离，它报告的平均距离之间的建设性和破坏性的干涉在图像平面，计算和比较与像素大小，和空间相关函数的距离依赖性的推导。随附的文章在这个问题上描述了动态光散射显微镜的实验实施。
The theoretical basis of an optical microscope technique to image dynamically scattered light fluctuation decay rates (dynamic light scattering microscopy) is developed. It is shown that relative motions between scattering centers even smaller than the optical resolution of the microscope are sufficient to produce significant phase variations resulting in interference intensity fluctuations in the image plane. The timescale and time dependence for the temporal autocorrelation function of these intensity fluctuations is derived. The spatial correlation distance, which reports the average distance between constructive and destructive interference in the image plane, is calculated and compared with the pixel size, and the distance dependence of the spatial correlation function is derived. The accompanying article in this issue describes an experimental implementation of dynamic light scattering microscopy.