Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator
Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator
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基于空间光调制器的可寻址多区域和多焦点多光子显微镜
DOI：
10.1117/1.jbo.17.3.030505
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发表时间：
2012-03-01
影响因子：
3.5
通讯作者：
Gao, Bruce Z.
中科院分区：
文献类型：
--
作者：
Shao, Yonghong;Qin, Wan;Gao, Bruce Z.
通过将偏转纯相位衍射空间光调制器 (SLM) 和振镜扫描仪相结合，开发出了可寻址的多区域和多焦点多光子显微镜 (AM-MMM)。 SLM 将传入的锁模近红外钛蓝宝石激光束整形为多个子束阵列，这些子束阵列具有可寻址的形状和尺寸，与样品上的感兴趣区域相匹配。与传统的多焦点多光子显微镜（MMM）相比，AM-MMM在不牺牲分辨率的情况下实现了激光功率的有效利用，提高了成像速率，减少了光损伤。 (C) 2012 年光电仪器工程师协会 (SPIE)。 [DOI：10.1117/1.JBO.17.3.030505]
Through a combination of a deflective phase-only diffractive spatial light modulator (SLM) and galvo scanners, an addressable multiregional and multifocal multiphoton microscope (AM-MMM) is developed. The SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple beamlet arrays with addressable shapes and sizes that match the regions of interest on the sample. Compared with conventional multifocal multiphoton microscope (MMM), AM-MMM achieves the effective use of the laser power with an increase of imaging rate and a decrease of photodamage without sacrifice of resolution. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.3.030505]