Qualitative and quantitative studies of cytokines synthesized and secreted by non-human primate peripheral blood mononuclear cells.
Qualitative and quantitative studies of cytokines synthesized and secreted by non-human primate peripheral blood mononuclear cells.
复制标题
非人灵长类动物外周血单核细胞合成和分泌的细胞因子的定性和定量研究。
DOI：
10.1016/1043-4666(93)90038-7
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发表时间：
1993
期刊：
影响因子：
3.8
通讯作者：
Ansari,AA
中科院分区：
文献类型：
--
作者：
Villinger,F;Hunt,D;Mayne,A;Vuchetich,M;Findley,H;Ansari,AA
非人灵长类动物正被用于各种临床前研究，包括那些涉及器官移植排斥机制的研究，以及那些被用作测试疫苗对各种传染病的效力的模型，最明显的是艾滋病。这些研究显然涉及免疫效应机制，包括T细胞、B细胞、单核细胞之间的相互作用，以及调节这些相互作用的细胞因子。然而，对非人类灵长类动物细胞因子的检测和定量知之甚少。为了解决这一问题，人们评估了生物检测、商品化的EIA试剂盒以及针对人类细胞因子的特异性引物对和探针的检测和定量能力。数据表明，尽管检测人IL-1α、干扰素-γ和肿瘤坏死因子-β的试剂盒失败，但检测IL-1β、IL-2、IL-4、IL-6和肿瘤坏死因子-α的试剂盒、每种细胞因子的生物测定和RT-PCR法都成功地检测并很可能定量了非人类灵长类细胞因子的同系物。这些测试将极大地促进未来对细胞因子在这些非人类灵长类研究中的作用的研究。
Non-human primates are being utilized in a variety of pre-clinical studies, including those involved with mechanisms of organ transplant rejection and those being used as models to test the efficacy of vaccines against a variety of infectious diseases, most notably AIDS. These studies clearly involve immunological effector mechanisms, which include the interaction between T cells, B cells, monocytes, and cytokines that regulate these interactions. However, there is very little known about assays and quantitation of cytokines from non-human primates.In attempts to address this issue, bioassays, commercially available EIA kits, and primer pairs and probes specific for human cytokines were evaluated for their ability to detect and quantitate the non-human primate homologues. Data suggest that although the EIA kits that were evaluated for human IL-1α, IFN-γ, and TNF-β failed, the EIA kits for IL-1β, IL-2, IL-4, IL-6, and TNF-α, the bioassays and RT-PCR assays for each of the cytokines were successful in detection and most likely quantitation of the non-human primate cytokine homologues. These assays will greatly facilitate future studies on the role of cytokines in these non-human primate studies.