Investigation of nitrogen-fixing potential in soil bacterial microbiota from Lapland boreal forest limit.
Investigation of nitrogen-fixing potential in soil bacterial microbiota from Lapland boreal forest limit.
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发表时间：
2010
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通讯作者：
Shintaro Hara;T. Tahvanainen;Y. Hashidoko;R. Gilkes;N. Prakongkep
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作者：
Shintaro Hara;T. Tahvanainen;Y. Hashidoko;R. Gilkes;N. Prakongkep
在高纬度或高海拔的森林生态系统中，虽然固氮细菌在森林床土中可能起着重要的供氮作用，但其氮循环的研究还不清楚。本研究以拉普兰帕拉斯（Pallas，Lapland）北方高纬度地区（68 ° N，森林界限）土壤为研究对象，考察了培养条件如培养基碳源浓度和温度对土壤细菌固氮的影响。虽然这些细菌微生物区系没有表现出任何固氮在培养基中含有1.0%的碳源，浓度被广泛用于根瘤菌和其他固氮菌，拉普兰土壤表现出固氮能力，在相对较低的碳源浓度（0.30.02%）。从土壤和培养的细菌中提取DNA，这些细菌在交替条件下在贫氮培养基中孵育。采用变性梯度凝胶电泳（DGGE）技术，对高纬度林限区土壤细菌群落进行分析，以揭示土壤固氮细菌群落的特征。
In forest ecosystem of high latitude or high elevation, the nitrogen cycle has not been elucidated, although nitrogen-fixing bacteria may play an important role in forest bed soil for nitrogen supply. In this study, bacterial nitrogen fixation in soil of Northern high latitude area in Pallas, Lapland (68˚N, forest limit) was investigated, and effective of incubation conditions for nitrogen fixation such as concentration of carbon source in medium and temperature, were examined. Although these bacterial microflora did not show any nitrogen fixation in a medium containing 1.0% carbon source, a concentration that is widely used for rhizobia and other diazotrophs, the Lapland soil showed nitrogen-fixing abilities at relatively low (0.30.02%) concentration of carbon source. DNA was extracted from the soil and from cultured bacteria which were incubated in nitrogen-poor medium under alternative conditions. Using DGGE (denaturing gradient gel electrophoresis) technique, analyses for bacteria community of soil of high latitude forest limit area is undertaken in order to reveal specific features of the nitrogen-fixing bacterial community.