The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species
The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species
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DOI：
10.1126/science.aap8328
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发表时间：
2018-05-11
期刊：
影响因子：
56.9
通讯作者：
Capel, Blanche
中科院分区：
文献类型：
--
作者：
Ge, Chutian;Ye, Jian;Capel, Blanche
温度依赖性性别决定是一个值得注意的表型可塑性模型。在许多爬行动物中，包括红耳滑龟Trachemys scripta elegans（T。在卵孵化期间，个体的性别由环境温度决定。在这项研究中，我们发现组蛋白H3赖氨酸27（H3K27）脱甲基酶KDM6B在T.在性腺之前的scripta胚胎是不同的。在26摄氏度（所有后代都发育成雄性的温度）下敲除Kdm6b会触发>80%的存活胚胎发生雄性到雌性的性别逆转。KDM6B通过消除其启动子附近H3K27的三甲基化直接促进雄性性别决定基因Dmrt 1的转录。此外，Dmrt 1的过表达足以挽救由Kdm6b破坏诱导的性逆转。这项研究建立了因果关系和直接的遗传联系的表观遗传机制和温度依赖性性别决定的海龟物种。
Temperature-dependent sex determination is a notable model of phenotypic plasticity. In many reptiles, including the red-eared slider turtle Trachemys scripta elegans (T. scripta), the individual's sex is determined by the ambient temperature during egg incubation. In this study, we show that the histone H3 lysine 27 (H3K27) demethylase KDM6B exhibits temperature-dependent sexually dimorphic expression in early T. scripta embryos before the gonad is distinct. Knockdown of Kdm6b at 26 degrees C (a temperature at which all offspring develop into males) triggers male-to-female sex reversal in >80% of surviving embryos. KDM6B directly promotes the transcription of the male sex-determining gene Dmrt1 by eliminating the trimethylation of H3K27 near its promoter. Additionally, overexpression of Dmrt1 is sufficient to rescue the sex reversal induced by disruption of Kdm6b. This study establishes causality and a direct genetic link between epigenetic mechanisms and temperature-dependent sex determination in a turtle species.