Testing four barcoding markers for species identification of Potamogetonaceae
Testing four barcoding markers for species identification of Potamogetonaceae
复制标题
测试用于眼子菜科物种鉴定的四种条形码标记
DOI：
10.1111/j.1759-6831.2011.00131.x
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发表时间：
2011-05-01
影响因子：
3.7
通讯作者：
Wang, Qing-Feng
中科院分区：
文献类型：
--
作者：
Du, Zhi-Yuan;Qimike, Alitong;Wang, Qing-Feng
眼子菜科（Potamogetonaceae）是水生环境中最重要的植物类群之一。由于其高度的形态和生态多样性，这一水生家庭的物种识别仍然存在问题。DNA条形码涉及对标准DNA区域进行测序，已被证明是物种鉴定的有力工具。在本研究中，我们测试了4个条形码标记（rbcL，matK，内转录间隔区（ITS），和trnH-psbA）在15眼子菜属物种和两个Stuckenia物种，代表了大多数种眼子菜科在中国。结果表明，这4个地区都能区分并支持新提出的属属-利用ITS和trnH-psbA分析表明，种间存在显著的遗传变异。然而，trnH-psbA的种内遗传变异性较高，因此不适合用于眼子菜科的条形码编码。ITS区和matK区具有很好的区分性。然而，matK不易使用通用引物测序。ITS是表现最好的单基因座，使其成为眼子菜科中潜在有用的DNA条形码。
The pondweeds (Potamogetonaceae) are among the most important plant groups in the aquatic environment. Owing to their high morphological and ecological diversity, species identification of this aquatic family remains problematic. DNA barcoding involves sequencing a standard DNA region and has been shown to be a powerful tool for species identification. In the present study, we tested four barcoding markers (rbcL, matK, internal transcribed spacer (ITS), and trnH-psbA) in 15 Potamogeton species and two Stuckenia species, representing most species of the Potamogetonaceae in China. The results show that all four regions can distinguish and support the newly proposed genera of Stuckenia from Potamogeton. Using ITS and trnH-psbA, significant interspecific genetic variability was shown. However, intraspecific genetic variability of trnH-psbA is high and so it is not suitable for barcoding in Potamogetonaceae. The ITS and matK regions showed good discrimination. However, matK was not easy to sequence using universal primers. The best performing single locus was ITS, making it a potentially useful DNA barcode in Potamogetonaceae.