Multiomics analysis of kernel development in response to short-term heat stress at the grain formation stage in waxy maize
Multiomics analysis of kernel development in response to short-term heat stress at the grain formation stage in waxy maize
复制标题
糯玉米籽粒形成阶段短期热胁迫下籽粒发育的多组学分析
DOI：
10.1093/jxb/erab286
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发表时间：
2021-06-15
影响因子：
6.9
通讯作者：
Lu, Dalei
中科院分区：
文献类型：
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作者：
Guo, Jian;Gu, Xiaotian;Lu, Dalei
了解玉米籽粒形成期高温胁迫下籽粒的适应性变化，对于制定减轻高温胁迫对产量和品质的负面影响的策略至关重要。本研究以糯玉米（Zea mays L.）结果表明：（1）在授粉后1-15 d，正常日/正常夜（对照）、高温日/正常夜、正常日/高温夜、高温日/高温夜4种温度条件下，授粉后1-15 d，正常日/正常夜（对照）、高温日/正常夜（高温日/高温夜）、高温日/高温夜（高温日/高温夜）4种温度条件下，授粉后1-15 d，正常日/正常夜（对照）、高温日/高温夜（高温日/高温夜）、高温日/高温夜（高温日/高温夜）4种温度条件下，授粉后1-15 d，正常日/正常夜（对照）、高温日/高温夜（高温日/高温夜）、高温日/高温夜（高温日/高温夜）和高温日/高温夜（高温日/高温夜）4种温度条件下，授粉后1-15 d，正常日/高温夜（对照）和高温日/高温夜（高温日/高温夜）4种温度条件下，授粉后1-15 d，正常日/与对照相比，3种高温处理均抑制了籽粒发育和淀粉沉积。为了了解籽粒对高温胁迫的反应，在10 DAP和25 DAP研究了它们的转录组、蛋白质组和代谢组。这表明，与籽粒发育和淀粉沉积相关的基因和蛋白质在10 DAP分别上调和下调，但这种模式在25 DAP被逆转。高温胁迫下的代谢物组分析表明，在10 DAP和25 DAP的代谢产物的积累模式呈负相关。我们的多组学分析表明，生长素，脱落酸和水杨酸介导的信号通路对高温胁迫的反应在10 DAP比在25 DAP更活跃。这些结果证实了籽粒发育早期的高温胁迫对后期的发育具有一定的延续效应。总之，我们在高温胁迫下发育的籽粒的多组学谱提供了对高温条件下玉米产量和质量的基础过程的见解。
Understanding the adaptive changes in maize kernels under high-temperature stress during grain formation stage is critical for developing strategies to alleviate the negative effects on yield and quality. In this study, we subjected waxy maize (Zea mays L. sinensis Kulesh) to four different temperature regimes from 1-15 d after pollination (DAP), namely normal day/normal night (control), hot day/normal night, normal day/hot night, and hot day/hot night. Compared to the control, the three high-temperature treatments inhibited kernel development and starch deposition. To understand how the kernels responded to high-temperature stress, their transcriptomes, proteomes, and metabolomes were studied at 10 DAP and 25 DAP. This showed that genes and proteins related to kernel development and starch deposition were up- and down-regulated, respectively, at 10 DAP, but this pattern was reversed at 25 DAP. Metabolome profiling under high-temperature stress showed that the accumulation patterns of metabolites at 10 DAP and 25 DAP were inversely related. Our multiomics analyses indicated that the response to high-temperature stress of signaling pathways mediated by auxin, abscisic acid, and salicylic acid was more active at 10 DAP than at 25 DAP. These results confirmed that high-temperature stress during early kernel development has a carry-over effect on later development. Taken together, our multiomics profiles of developing kernels under high-temperature stress provide insights into the processes that underlie maize yield and quality under high-temperature conditions.