Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels
Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels
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DOI：
10.1021/ac990674w
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发表时间：
1999-11-01
影响因子：
7.4
通讯作者：
Novotny, MV
中科院分区：
文献类型：
--
作者：
Chen, P;Werner-Zwanziger, U;Novotny, MV
在正离子模式下的基质辅助激光解吸/电离(MALDI)质谱分析中，发现过苯甲酰化唾液酸寡糖是稳定的衍生物，给出了强烈的信号。末端Neu5NAcα2-gt；3和α2-gt；6Gal单元的寡糖在苯甲酰化过程中经历了特有的结构变化，产生了容易识别的质谱图。亚皮考摩尔碳水化合物样品成功地进行了苯甲酰化，并用MALDI质谱仪进行了分析。
Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAc alpha 2-->3 and alpha 2-->6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.