Th1-, Th2-, and Th17-Related Cytokine and Chemokine Receptor mRNA and Protein Expression in the Brain Tissues, T Cells, and Macrophages of DogsWith Necrotizing and Granulomatous Meningoencephalitis
Th1-, Th2-, and Th17-Related Cytokine and Chemokine Receptor mRNA and Protein Expression in the Brain Tissues, T Cells, and Macrophages of DogsWith Necrotizing and Granulomatous Meningoencephalitis
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DOI：
10.1177/0300985813488957
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发表时间：
2013-11-01
影响因子：
2.4
通讯作者：
Nakayama, H.
中科院分区：
文献类型：
--
作者：
Park, E. -S.;Uchida, K.;Nakayama, H.
坏死性脑膜脑炎（NME）、坏死性白质脑炎（NLE）和肉芽肿性脑膜脑脊髓炎（GME）是犬中枢神经系统（CNS）的特发性炎症性疾病。在我们之前的研究中，除CD3阳性T细胞外，炎症细胞的比例在脑实质和血管周围病变中没有差异。然而，这些疾病的品种特异性、临床病程和具体病变是不同的。因此，暗示了这些疾病的病理学的相似性和差异。本研究对 NME (n = 2)、NLE (n = 4) 和 GME (n = 2) 病例中细胞因子和趋化因子受体的信使 RNA (mRNA) 和/或蛋白表达水平进行了研究，并讨论了它们在特定病变形成中的关系。干扰素 (IFN)-和白细胞介素 (IL)-17 的 mRNA 和蛋白表达水平分别在 NME 和 GME 中标记。 CXCR3 和 CCR2 的 mRNA 表达水平也分别在 NME 和 GME 中标记。用于识别这些病变中产生 IL-17 的细胞的双标记免疫荧光结果表明，GME 中大多数 CD163 阳性巨噬细胞/小胶质细胞但较少 CD3 阳性 T 细胞呈 IL-17 阳性。这些结果表明，IFN-γ在 NME 病变中发挥着关键作用，并且浸润脑病变并产生 IL-17 的巨噬细胞/小胶质细胞在 GME 中比 T 细胞更重要。
Necrotizing meningoencephalitis (NME), necrotizing leukoencephalitis (NLE), and granulomatous meningoencephalomyelitis (GME) are idiopathic inflammatory diseases in the central nervous system (CNS) of dogs. In our previous study, the proportion of inflammatory cells, except for CD3-positive T cells, were not different in parenchymal and perivascular lesions in the brain. However, breed specificities, clinical courses, and specific lesions were distinct among these diseases. Thus, similarities and differences in the pathologies of these diseases have been implied. In this study, the messenger RNA (mRNA) and/or protein expression levels of cytokines and chemokine receptors were investigated in NME (n = 2), NLE (n = 4), and GME (n = 2) cases, and their relationship in the formation of specific lesions was discussed. The mRNA and protein expression levels of interferon (IFN)- and interleukin (IL)-17 were marked in NME and GME, respectively. The mRNA expression levels of CXCR3 and CCR2 were also marked in NME and GME, respectively. The results of double-labeling immunofluorescence, used to identify cells producing IL-17 in these lesions, showed that most CD163-positive macrophages/microglia but fewer CD3-positive T cells were IL-17 positive in GME. These results indicate that IFN- plays a key role in NME lesions and that the macrophages/microglia that infiltrate brain lesions producing IL-17 are more important in GME than T cells.