IN VITRO ASSESSMENT OF ANTIOXIDANT, PHYTOCHEMICAL AND NUTRITIONAL PROPERTIES OF EXTRACTS FROM THE LEAVES OF OCIMUM GRATISSIMUM (LINN)
IN VITRO ASSESSMENT OF ANTIOXIDANT, PHYTOCHEMICAL AND NUTRITIONAL PROPERTIES OF EXTRACTS FROM THE LEAVES OF OCIMUM GRATISSIMUM (LINN)
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DOI：
10.4314/ajtcam.v10i5.11
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Emuedo, Oke A.
中科院分区：
文献类型：
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作者：
Igbinosa, Etinosa O.;Uzunuigbe, Edwina O.;Emuedo, Oke A.
研究了丙酮、甲醇和罗勒叶水提取物的抗氧化、植物化学和营养特性，以评估该植物叶子的治疗和营养潜力。根据 1,1-二苯基-2-三硝基苯肼 (DPPH) 和 2,2-偶氮双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐 (ABTS) 和铁还原剂评估植物提取物的抗氧化剂。测定总酚、类黄酮、黄酮醇和原花青素，以评估它们对该植物抗氧化活性的相应影响。提取物表现出 DPPH 和 ABTS(.+) 自由基清除活性，与丁基羟基甲苯 (BHT) 相当。植物化学筛选显示存在生物碱、单宁、皂苷、类固醇、强心苷、类黄酮、萜类化合物和苯酚。初步分析证实，叶子含有大量的灰分、粗蛋白、脂质、纤维和碳水化合物。常量和微量元素及成分显示，叶子含有大量的钠、钾、钙、镁、铁、锌、磷、铜、氮和锰。这项研究表明，叶子可以用作治疗剂，并证明其在民俗医学中的应用是合理的。
The antioxidant, phytochemical and nutritional properties of acetone, methanol and aqueous extracts of the leaves of Ocimum gratissimum (Linn) were investigated to evaluate the therapeutic and nutritional potential of the leaves of this plant. The antioxidant of the plant extracts were assessed against 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing agent. Total phenolics, flavonoids, flavonols and proanthocyanidins were determined to assess their corresponding effect on the antioxidant activity of this plant. The extracts exhibited DPPH and ABTS(.+) radical scavenging activities, which was comparable to butylated hydroxytoluene (BHT). The phytochemical screening revealed the presence of alkaloids, tannins, saponin, steroids, cardiacglycoside, flavonoid, terpenoids and phenol. The proximate analysis confirms that the leaves contain appreciable amount of ash, crude protein, lipids, fibre and carbohydrates. The macro and micro elements and constituents revealed that the leaves contain significant amount of sodium, potassium, calcium, magnesium, iron, zinc, phosphorus, copper, nitrogen, and manganese. This study shows that the leaf can be used as a therapeutic agent and justifies its application in folkloric medicine.