SIMR foci are found in the progenitor germ cells of C. elegans embryos.
SIMR foci are found in the progenitor germ cells of C. elegans embryos.
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DOI：
10.17912/micropub.biology.000374
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发表时间：
2021-02-22
影响因子：
--
通讯作者：
Phillips CM
中科院分区：
文献类型：
--
作者：
Uebel CJ;Manage KI;Phillips CM
RNA干扰是真核生物中广泛保守的基因调控和沉默机制。In C.在线虫中，RNA沉默通过核周核内至少含有4个颗粒：P颗粒、Z颗粒、突变灶和SIMR灶。这些颗粒的胚胎定位是已知的，除了SIMR病灶。在这里，我们建立了SIMR灶首先出现在P4生殖系卵裂球的核周边，并成为众多和明亮的Z2和Z3祖生殖细胞。这一时间与其他生殖细胞颗粒的出现或分层相吻合，为协调的生殖细胞颗粒重组提供了进一步的证据。
RNA interference is a widely conserved mechanism of gene regulation and silencing across eukaryotes. In C. elegans, RNA silencing is coordinated through perinuclear nuage containing at least four granules: P granules, Z granules, Mutator foci, and SIMR foci. Embryonic localization of these granules is known for all except SIMR foci. Here we establish that SIMR foci first appear at the nuclear periphery in the P4 germline blastomere and become numerous and bright in the Z2 and Z3 progenitor germ cells. This timing coincides with the appearance or de-mixing of other germline granules, providing further evidence for coordinated germ granule reorganization.