ANAEROBIC BIODEGRADATION OF THE LIGNIN AND POLYSACCHARIDE COMPONENTS OF LIGNOCELLULOSE AND SYNTHETIC LIGNIN BY SEDIMENT MICROFLORA
ANAEROBIC BIODEGRADATION OF THE LIGNIN AND POLYSACCHARIDE COMPONENTS OF LIGNOCELLULOSE AND SYNTHETIC LIGNIN BY SEDIMENT MICROFLORA
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DOI：
10.1128/aem.47.5.998-1004.1984
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发表时间：
1984-01-01
影响因子：
4.4
通讯作者：
HODSON, RE
中科院分区：
文献类型：
--
作者：
BENNER, R;MACCUBBIN, AE;HODSON, RE
具体地，放射性标记的[14 C-木质素]木质纤维素和[14 C-多糖]木质纤维素由多种海洋和淡水湿地植物制备，包括草、莎草、灯心草和硬木。[互花米草（Spartina alterniflora）、苔草（Carex walkeriana）、灯心草（Juncus roemerianus）和红树（Rhizophora mangle）]。这些[14 C]木质纤维素制剂和合成的[14 C]木质素与从盐沼、淡水沼泽和红树林沼泽收集的缺氧沉积物一起厌氧培养。在长达300天的长期培养过程中，木质纤维素的木质素和多糖组分缓慢厌氧降解为14 CO2和14 CH 4。来自草本植物的木质纤维素比来自硬木的木质纤维素降解得更快。294天后，16.9%的木质素组分和30.0%的来自所用草的木质纤维素多糖组分（S.互花米草）降解为气态终产物。相比之下，在246天后，仅1.5%的木质素组分和4.1%的来自所用硬木的木质纤维素的多糖组分（R. mangle）降解为气态终产物。合成[14 C]木质素厌氧降解速度比硬木木质纤维素的木质素组分; 276天后，3.7%的合成木质素降解为气态终产物。与以前的报道相反，这些结果表明，木质素和木质素植物组织在没有O2的情况下是可生物降解的。尽管木质纤维素是厌氧生物降解的抑制剂，但在水生沉积物中测得的降解速率是显著的，并且对来自这些丰富的生物聚合物的C的生物圈循环具有重要意义。
Specifically radiolabeled [14C-lignin]lignocelluloses and [14C-polysaccharide]lignocelluloses were prepared from a variety of marine and freshwater wetland plants including a grass, a sedge, a rush and a hardwood. [Spartina alterniflora, Carex walkeriana, Juncus roemerianus and Rhizophora mangle, respectively]. These [14C]lignocellulose preparations and synthetic [14C]lignin were incubated anaerobically with anoxic sediments collected from a salt marsh, a freshwater marsh and a mangrove swamp. During long-term incubations lasting up to 300 days, the lignin and polysaccharide components of the lignocelluloses were slowly degraded anaerobically to 14CO2 and 14CH4. Lignocelluloses derived from herbaceous plants were degraded more rapidly than lignocellulose derived from the hardwood. After 294 days, 16.9% of the lignin component and 30.0% of the polysaccharide component of lignocellulose derived from the grass used (S. alterniflora) were degraded to gaseous end products. In contrast, after 246 days, only 1.5% of the lignin component and 4.1% of the polysaccharide component of lignocellulose derived from the hardwood used (R. mangle) were degraded to gaseous end products. Synthetic [14C]lignin was degraded anaerobically faster than the lignin component of the hardwood lignocellulose; after 276 days, 3.7% of the synthetic lignin was degraded to gaseous end products. Contrary to previous reports, these results demonstrate that lignin and lignified plant tissues are biodegradable in the absence of O2. Although lignocelluloses are recalcitrant to anaerobic biodegradation, rates of degradation measured in aquatic sediments are significant and have important implications for the biospheric cycling of C from these abundant biopolymers.