High‐resolution axial and lateral position sensing using two‐photon excitation of fluorophores by a continuous‐wave Nd:YAG laser
High‐resolution axial and lateral position sensing using two‐photon excitation of fluorophores by a continuous‐wave Nd:YAG laser
复制标题
使用连续波 Nd:YAG 激光器对荧光团进行双光子激发进行高分辨率轴向和横向位置传感
DOI：
10.1063/1.118134
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发表时间：
1996
影响因子：
4
通讯作者：
E. Stelzer
中科院分区：
文献类型：
--
作者：
E. Florin;J. Hörber;E. Stelzer
使用连续波Nd：YAG激光器（λ=1064 nm）的双光子吸收诱导的荧光发射监测光镊聚焦体积内捕获的荧光珠的位置变化。由于与其环境的相互作用，珠的位移导致用于设计新型空间传感器的荧光强度变化。我们测定了直径为1.03 μm的CY 3标记乳胶珠的轴向位置变化，精度优于10 nm。在600 mW/ μm2的强度下，双光子漂白率低于50%/2000 s。
The change in position of fluorescent beads captured inside the focal volume of optical tweezers is monitored using fluorescence emission induced by two‐photon absorption of a continuous‐wave Nd:YAG laser (λ=1064 nm). The displacement of a bead due to interactions with its environment leads to a fluorescence intensity variation that is used to design a novel spatial sensor. We determine changes in the axial position of a CY3‐labeled latex bead with a diameter of 1.03 μm to a precision better than 10 nm. At an intensity of 600 mW/ μm2 the two‐photon bleaching rate is lower than 50% per 2000 s.