Trophic stimulation of cultured neurons from neonatal rat brain by epidermal growth factor.
Trophic stimulation of cultured neurons from neonatal rat brain by epidermal growth factor.
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DOI：
10.1126/science.3498986
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发表时间：
1987-10
期刊：
影响因子：
56.9
通讯作者：
R. Morrison;H. Kornblum;F. Leslie;R. Bradshaw
中科院分区：
文献类型：
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作者：
R. Morrison;H. Kornblum;F. Leslie;R. Bradshaw
表皮生长因子（Epidermal growth factor，EGF）是一种从成年雄性小鼠颌下腺中提取的多肽类促分裂剂。它也作为一种胃肠激素。EGF免疫反应物质最近已被确定在啮齿动物脑神经纤维和终端。在本研究中，发现EGF以剂量依赖性方式增强新生大鼠大脑新皮质下端脑神经元原代培养物的存活和过程生长。这种效应在EGF浓度低至每毫升100皮克（0.016纳摩尔）时观察到，并且依赖于培养基中EGF的持续存在。用碱性成纤维细胞生长因子也观察到类似的作用，但其他几种生长促进物质，包括神经胶质细胞的其他有丝分裂原，没有作用。因此，表皮生长因子，除了其促有丝分裂和激素的活动，可以作为一个神经突起的延伸和维护因素选择啮齿动物中枢神经系统的神经元。
Epidermal growth factor (EGF) is a potent polypeptide mitogen originally isolated from the adult male mouse submaxillary gland. It also acts as a gastrointestinal hormone. EGF-immunoreactive material has recently been identified within neuronal fibers and terminals in rodent brain. In the present study, EGF was found to enhance survival and process outgrowth of primary cultures of subneocortical telencephalic neurons of neonatal rat brain in a dose-dependent manner. This effect was observed with EGF concentrations as low as 100 picograms per milliliter (0.016 nanomolar) and was dependent on the continuous presence of EGF in the medium. Similar effects were observed with basic fibroblast growth factor, but several other growth-promoting substances, including other mitogens for glial elements, were without effect. Thus EGF, in addition to its mitogenic and hormonal activities, may act as a neurite elongation and maintenance factor for select neurons of the rodent central nervous system.