ISOLATION AND PROPERTIES OF A TEMPERATURE-SENSITIVE MUTANT (TS-412) OF AN INFLUENZA-A VIRUS RECOMBINANT WITH A TS LESION IN THE GENE CODING FOR THE NONSTRUCTURAL PROTEIN
ISOLATION AND PROPERTIES OF A TEMPERATURE-SENSITIVE MUTANT (TS-412) OF AN INFLUENZA-A VIRUS RECOMBINANT WITH A TS LESION IN THE GENE CODING FOR THE NONSTRUCTURAL PROTEIN
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DOI：
10.1016/0042-6822(81)90003-9
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发表时间：
1981-01-01
期刊：
影响因子：
3.7
通讯作者：
SCHOLTISSEK, C
中科院分区：
文献类型：
--
作者：
KOENNECKE, I;BOSCHEK, CB;SCHOLTISSEK, C
将鸡瘟病毒（FPV）与N病毒重组体经诱变处理后，分离到一株温度敏感突变株（ts 412），该突变株编码非结构蛋白（NS）的基因存在缺陷。这种突变体的特点是在生物学方面。NS蛋白是早期蛋白，但其412的病变是晚期缺陷。在非允许温度下，除血凝素和M蛋白产量相对较低外，所有病毒产物均以正常产量合成。在40 ℃下不形成颗粒。C.在这些条件下，在感染ts 412的鸡胚细胞的细胞质中发现了电子致密包涵体。这些结构可能是NS蛋白之一的积累。
After mutagenizing a recombinant between fowl plague virus (FPV) and virus N a temperature-sensitive (ts) mutant (ts 412) was isolated which carried a defect in the gene coding for the nonstructural (NS) proteins. This mutant was characterized in biological terms. The NS proteins are early proteins, but the lesion of its 412 is a late defect. At the nonpermissive temperature all viral products are synthesized in normal yields except for hemagglutinin and M protein which are produced in relatively low amounts. No particles are formed at 40.degree. C. Electron-dense inclusions were found under these conditions in the cytoplasm of chick embryo cells infected with ts 412. The structures may be accumulations of one of the NS proteins.