Expression profiling identifies genes expressed early during lint fibre initiation in cotton.
Expression profiling identifies genes expressed early during lint fibre initiation in cotton.
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DOI：
10.1093/pcp/pci228
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发表时间：
2006
影响因子：
4.9
通讯作者：
Yingru Wu;A. Machado;R. White;D. Llewellyn;E. Dennis
中科院分区：
文献类型：
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作者：
Yingru Wu;A. Machado;R. White;D. Llewellyn;E. Dennis
棉纤维是单个表皮细胞的一个子集，从种皮延伸出来，产生长的纤维素股或用于纺纱的棉绒。为了确定基因，可能会调节皮棉纤维的起始，花后0天（DPA）的整个胚珠从6个减少纤维或无毛突变体的表达谱进行了比较与野生型棉绒棉花使用cDNA微阵列。许多克隆的差异表达，但当只有那些基因，通常表达在胚珠外珠被（纤维发展）被认为是，只有13个不同的cDNA克隆下调的一些或所有的突变体。其中包括：与金鱼草Myb AmMIXTA相似的Myb转录因子（GhMyb 25），一个推定的同源结构域蛋白（与拟南芥ATML 1相关），一个细胞周期蛋白D基因，一些先前鉴定的纤维表达的结构和代谢基因，如脂质转移蛋白、α-扩张蛋白和蔗糖合成酶，以及一些未知的基因。激光捕获显微切割和逆转录-PCR显示，GhMyb 25和同源结构域基因主要是胚珠特异性的，并在开花当天在纤维初始相对于相邻的非纤维胚珠表皮细胞上调。因此，它们的空间和时间表达模式与纤维起始的时间和位置相吻合。GhMyb 25在转基因烟草中的组成型过表达导致分枝的长柄叶毛状体的增加。细胞周期基因的参与促使DNA含量的测量表明，纤维的初始，如叶毛状体，进行DNA核内复制。因此，棉纤维的起始与叶毛状体的发育有一些相似之处，尽管详细的分子机制明显不同。
Cotton fibres are a subset of single epidermal cells that elongate from the seed coat to produce the long cellulose strands or lint used for spinning into yarn. To identify genes that might regulate lint fibre initiation, expression profiles of 0 days post-anthesis (dpa) whole ovules from six reduced fibre or fibreless mutants were compared with wild-type linted cotton using cDNA microarrays. Numerous clones were differentially expressed, but when only those genes that are normally expressed in the ovule outer integument (where fibres develop) were considered, just 13 different cDNA clones were down-regulated in some or all of the mutants. These included: a Myb transcription factor (GhMyb25) similar to the Antirrhinum Myb AmMIXTA, a putative homeodomain protein (related to Arabidopsis ATML1), a cyclin D gene, some previously identified fibre-expressed structural and metabolic genes, such as lipid transfer protein, alpha-expansin and sucrose synthase, as well as some unknown genes. Laser capture microdissection and reverse transcription-PCR were used to show that both the GhMyb25 and the homeodomain gene were predominantly ovule specific and were up-regulated on the day of anthesis in fibre initials relative to adjacent non-fibre ovule epidermal cells. Their spatial and temporal expression pattern therefore coincided with the time and location of fibre initiation. Constitutive overexpression of GhMyb25 in transgenic tobacco resulted in an increase in branched long-stalked leaf trichomes. The involvement of cell cycle genes prompted DNA content measurements that indicated that fibre initials, like leaf trichomes, undergo DNA endoreduplication. Cotton fibre initiation therefore has some parallels with leaf trichome development, although the detailed molecular mechanisms are clearly different.