DO CELLS CYCLE
DO CELLS CYCLE
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DOI：
10.1073/pnas.70.4.1263
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
MARTIN, L
中科院分区：
文献类型：
--
作者：
SMITH, JA;MARTIN, L
我们认为细胞的生命分为两个根本不同的部分。有丝分裂后一段时间，所有细胞进入一种状态（A），在这种状态下，它们的活性不指向复制。细胞可以在任何时间长度内保持在A状态，在此期间，它离开A状态的概率保持恒定。在离开A状态时，细胞进入B期，其中它们的活动是确定性的，并且指向复制。因此，细胞复制过程的启动是随机的，就像放射性衰变是随机的一样。细胞群体生长速率由细胞离开A状态的概率、B期的持续时间和细胞死亡速率决定。这些参数的知识允许精确计算群体内的细胞分裂时间的分布、同步化细胞培养物的行为以及标记的有丝分裂曲线的形状。
We propose that a cell's life is divided into two fundamentally different parts. Some time after mitosis all cells enter a state (A) in which their activity is not directed towards replication. A cell may remain in the A-state for any lenght of time, throughout which its probability of leaving A-state remains constant. On leaving A-state, cells enter B-phase in which their activities are deterministic, and directed towards replication. Initiation of cell replication processes is thus random, in the sense that radioactive decay is random. Cell population growth rates are determined by the probability with which cells leave the A-state, the duration of the B-phase, and the rate of cell death. Knowledge of these parameters permits precise calculation of the distribution of intermitotic times within populations, the behavior of synchronized cell cultures, and the shape of labeled mitosis curves.