MicroRNA-500a-5p inhibits colorectal cancer cell invasion and epithelial-mesenchymal transition
MicroRNA-500a-5p inhibits colorectal cancer cell invasion and epithelial-mesenchymal transition
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MicroRNA-500a-5p抑制结直肠癌细胞侵袭和上皮间质转化
DOI：
10.3892/ijo.2020.5015
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发表时间：
2020-06-01
影响因子：
5.2
通讯作者：
Xiang, Li
中科院分区：
文献类型：
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作者：
Tang, Weimei;Hong, Linjie;Xiang, Li
恶性肿瘤的发展是一系列复杂的过程，其中大部分尚未阐明。本研究的目的是探讨影响结直肠癌细胞迁移和侵袭能力的microRNAs（miRNAs/miR）。我们先前的研究显示miR-500 a-5 p抑制结直肠癌细胞的生长和恶性转化。本研究表明，miR-500 a-5 p的过表达降低了波形蛋白的表达，同时增加了E-cadherin的表达。miR-500 a-5 p的抑制导致CRC细胞中梭形形态学变化和F-actin的重组。此外，miR-500 a-5 p减弱了EMT中的转化生长因子β信号通路。此外，大黄素抑制miR-500 a-5 p抑制剂并抑制EMT过程。在使用裸鼠的转移动物模型中，miR-500 a-5 p调节EMT和LoVo细胞转移。因此，本研究的发现表明，miR-500 a-5 p与CRC细胞的侵袭/迁移和间充质样细胞变化方面的积极治疗结果相关。
The development of malignant tumors is a series of complex processes, the majority of which have not been elucidated. The aim of the present study was to investigate the microRNAs (miRNAs/miR) that affect the migration and invasion abilities of CRC cells. Our previous reports have revealed that miR-500a-5p suppressed CRC cell growth and malignant transformation. The present study demonstrated that overexpression of miR-500a-5p reduced the expression of vimentin, while increasing the expression of E-cadherin. Inhibition of miR-500a-5p resulted in spindle-like morphological changes and reorganization of F-actin in CRC cells. Furthermore, miR-500a-5p attenuated the transforming growth factor-beta signaling pathway in EMT. Additionally, emodin inhibited the miR-500a-5p inhibitor and suppressed the EMT process. In animal models of metastasis using nude mice, EMT and LoVo cell metastasis was modulated by miR-500a-5p. Therefore, the findings of the present study demonstrated that miR-500a-5p is associated with a positive therapeutic outcome in terms of invasion/migration of CRC cells and mesenchymal-like cell changes.