Acute exercise-induced nitric oxide production contributes to upregulation of circulating endothelial progenitor cells in healthy subjects
Acute exercise-induced nitric oxide production contributes to upregulation of circulating endothelial progenitor cells in healthy subjects
复制标题
DOI：
10.1038/sj.jhh.1002171
复制
发表时间：
2007-06-01
影响因子：
2.7
通讯作者：
Tao, J.
中科院分区：
文献类型：
--
作者：
Yang, Z.;Wang, J-M;Tao, J.
运动已被证明可以促进人体循环内皮祖细胞（EPC）的数量和活性，有助于改善内皮功能和维持心血管稳态。然而，运动对健康受试者循环 EPC 产生影响的机制尚不完全清楚。在这里，我们研究了急性运动对循环 EPC 的调节是否与已知调节健康受试者循环 EPC 的一氧化氮 (NO)、血管内皮生长因子 (VEGF) 和粒细胞巨噬细胞集落刺激因子 (GM-CSF) 相关。共有 16 名健康男性志愿者接受了改良的 Bruce 跑步机急性运动方案。测量健康受试者运动前后循环EPCs的数量和活性，以及血浆和培养基中NO-VEGF和GM-CSF的水平。健康受试者急性运动后循环EPCs的数量和活性显着高于运动前。与此同时，急性运动显着提高了健康受试者的血浆一氧化氮水平。血浆NO水平升高与循环EPCs数量或活性增加之间存在显着的线性回归关系。然而，急性运动后血浆VEGF和GM-CSF水平没有观察到变化。培养的 EPC 分泌的 NO-VEGF 和 GM-CSF 对急性运动的反应保持不变。本研究首次证明，急性运动诱导的 NO 产生有助于健康受试者循环 EPC 的上调，这表明 NO 在运动对循环 EPC 的调节中发挥着重要作用。
Exercise has been proved to promote the number and activity of circulating endothelial progenitor cells (EPCs) in humans, which contributes to improvement in endothelial function and maintenance of cardiovascular homoeostasis. However, the mechanism underlying the effect of exercise on circulating EPCs in healthy subjects is not completely understood. Here, we investigated whether the regulation of acute exercise on circulating EPCs is associated with nitric oxide (NO), vascular endothelial growth factors (VEGF) and granulocyte macrophage colony stimulating factor (GM-CSF) known to modulate circulating EPCs in healthy subjects. A total of 16 healthy male volunteers underwent a modified Bruce treadmill acute exercise protocol. The number and activity of circulating EPCs, as well as the levels of NO-VEGF and GM-CSF in plasma and culture medium before and after exercise in healthy subjects were measured. The number and activity of circulating EPCs after acute exercise were significantly higher than those before exercise in healthy subjects. In parallel, acute exercise significantly enhanced plasma NO level in healthy subjects. There is a significant linear regression relationship between the enhanced plasma NO level and increased number or activity of circulating EPCs. However, no change of plasma VEGF and GM-CSF level was observed after acute exercise. The secretion of NO-VEGF and GM-CSF by cultured EPCs remained unchanged in response to acute exercise. The present study demonstrates for the first time that acute exercise-induced NO production contributes to upregulation of circulating EPCs in healthy subjects, which suggests that NO plays an important role in the regulation of exercise on circulating EPCs.