ESTABLISHMENT AND CHARACTERIZATION OF A MOUSE SCHWANN-CELL LINE WHICH PRODUCES MYELIN INVIVO
ESTABLISHMENT AND CHARACTERIZATION OF A MOUSE SCHWANN-CELL LINE WHICH PRODUCES MYELIN INVIVO
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DOI：
10.1002/jnr.490320103
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发表时间：
1992-05-01
影响因子：
4.2
通讯作者：
BARONVANEVERCOOREN, A
中科院分区：
文献类型：
--
作者：
BOUTRY, JM;HAUW, JJ;BARONVANEVERCOOREN, A
用大剂量血清从纯化的小鼠雪旺细胞培养中建立了雪旺细胞系(MSC-80)。MSC-80细胞系为非整倍体细胞系，倍增时间为17小时，已传代110多代。MSC-80细胞多为双极或星形(突起3-5个)。其他几个形状不规则，巨大，多核。所有MSC80细胞都表达髓鞘形成雪旺细胞的抗原，如S-100、224/58、层粘连蛋白和其他细胞外基质糖蛋白。然而，它们也表达非髓鞘形成的雪旺细胞抗原GFAP。通过延时摄影，MSC80细胞呈现出雪旺细胞特有的节律性波动。当被诱导在琼脂中形成聚集体时，它们形成细胞间连接和基底膜状结构。此外，在溶血磷脂诱导的损伤内或距离损伤处移植后，MSC80细胞在宿主脱髓鞘轴突周围形成髓鞘。因此，当在体外分离时，MSC80细胞表达一些正常的髓鞘形成雪旺细胞表型。此外，当它们与体内的轴突相关时，它们表现出形成髓鞘的主要优势。
A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3-5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin-forming Schwann cells such as S-100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the nonmyelin-forming Schwann cell antigen GFAP. By time-lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane-like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells form myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin-forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo.