Alleviation of heavy metal and silver nanoparticle toxicity and enhancement of their removal by hydrogen sulfide in Phanerochaete chrysosporium
Alleviation of heavy metal and silver nanoparticle toxicity and enhancement of their removal by hydrogen sulfide in Phanerochaete chrysosporium
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黄孢原毛平革菌重金属和纳米银颗粒毒性的减轻及硫化氢对其去除的增强
DOI：
10.1016/j.chemosphere.2019.02.190
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Chen Guiqiu
中科院分区：
文献类型：
--
作者：
Huang Zhenzhen;He Kai;Song Zhongxian;Zeng Guangming;Chen Anwei;Yuan Lei;Li Hui;Chen Guiqiu
硫化氢(H_2S)是一种重要的细胞信号分子，在介导对生物/非生物胁迫的反应中发挥着重要作用。研究了重金属和纳米银(AgNPs)对黄孢原毛平革菌(Phanerochaete Chrysosporium)金属去除、细胞活力和抗氧化反应的影响。随着硫化氢供体氢硫化钠(NaHS)浓度的增加，铅(ΙΙ)的去除效果增强，在100mgNaHS和200mgNaHS作用下，铅( )的最大脱除效率分别提高了31%和17%，加入500mgNaHS时，ΙΙ的最大去除效率提高了17%。在铅(II)胁迫下(10-200 μmg/L)，加入500 的M NaHS可使细胞存活率提高15%-39%。在AgNP胁迫下，NaHS还以浓度依赖的方式诱导总Ag摄取量和细胞存活率的增加。同时，在铅(II)、镉(II)、铜(II)、锌(II)、镍(II)和AgNPs胁迫下，NaHS的引入显著提高了超氧化物歧化酶和过氧化氢酶的活性。经NaHS处理后，黄孢子细胞的脂质过氧化和氧化应激受到抑制，这可能是由于抗氧化酶的上调所致。结果表明，硫化氢可以减轻重金属和AgNP对黄孢子菌的毒性，提高废水中这些有毒物质的去除效率。
Hydrogen sulfide (H2S), an important cellular signaling molecule, plays vital roles in mediating responses to biotic/abiotic stresses. Influences of H2S on metal removal, cell viability, and antioxidant response ofPhanerochaete chrysosporiumupon exposure to heavy metals and silver nanoparticles (AgNPs) in the present study were investigated. An enhancement in Pb(ΙΙ) removal with an increase in concentration of the H2S donor sodium hydrosulfide (NaHS) was observed, and the maximum removal efficiencies increased by 31% and 17% under 100 and 200 mg/L Pb(ΙΙ) exposure, respectively, in the presence of 500 μM NaHS. Application of 500 μM NaHS increased the cell viability by 15%–39% under Pb(II) stress (10–200 mg/L) with relative to the untreated control. Increase in total Ag uptake and cell survival was also elicited by NaHS in a concentration-dependent manner under AgNP stress. Meanwhile, activities of superoxide dismutase and catalase were significantly enhanced with the introduction of NaHS under stresses of Pb(II), Cd(II), Cu(II), Zn(II), Ni(II), and AgNPs. The inhibition in lipid peroxidation and oxidative stress was observed inP. chrysosporiumcells exposed to these toxicants following NaHS pretreatment, which could be attributed to the upregulation in antioxidant enzymes. The results obtained suggest that H2S can alleviate heavy metals and AgNP-induced toxicity toP. chrysosporiumand improve the removal efficiency of these toxicants from wastewater.