Influencing Mechanism of Ocean Acidification on Byssus Performance in the Pearl Oyster Pinctada fucata
Influencing Mechanism of Ocean Acidification on Byssus Performance in the Pearl Oyster Pinctada fucata
复制标题
海洋酸化对珠母贝足丝性能的影响机制
DOI：
10.1021/acs.est.7b02132
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发表时间：
2017-07-04
影响因子：
11.4
通讯作者：
Zhang, Rongqing
中科院分区：
文献类型：
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作者：
Li, Shiguo;Liu, Chuang;Zhang, Rongqing
足丝是双壳类在水下附着的重要结构。它容易受到二氧化碳驱动的海洋酸化（OA）的影响。以前的研究已经记录了OA对深海丝性能的显著不利影响，但其机制在很大程度上仍然未知。本研究采用多种方法探讨OA对合浦珠母贝足丝生长和力学性能影响的机制。结果表明，OA改变了足丝蛋白的丰度和二级结构，并影响了足丝远端金属离子的含量，导致足丝直径减小，纳米腔增大，导致机械性能（强度和延展性）降低。关键足蛋白基因的表达分析进一步证实了足丝蛋白丰度的变化。此外，比较转录组分析揭示了离子转运和足突相关类别的富集，足突相关途径的上调，以及“细胞外基质受体相互作用”途径的下调，这可能影响足运动生理学，导致足丝产生减少。本研究从机理上揭示了OA对珍珠牡蛎足丝的影响，有助于我们更全面地了解OA对海洋生态系统的影响。
The byssus is an important adhesive structure by which bivalves robustly adhere to underwater substrates. It is susceptible to carbon dioxide-driven ocean acidification (OA). Previous investigations have documented significant adverse effects of OA on the performance of byssal threads, but the mechanisms remain largely unknown. In this study, multiple approaches were employed to reveal the underlying mechanisms for the effects of OA on byssus production and mechanical properties in the pearl oyster Pinctada fucata. The results showed that OA altered the abundance and secondary structure of byssal proteins and affected the contents of metal ions in distal threads, which together reduced the byssus diameter and amplified byssus nanocavity) causing reductions in mechanical properties (strength and extensibility), Expression analysis of key foot protein genes further confirmed changes in byssal protein abundance. Moreover, comparative transcriptome analysis revealed enrichment of ion transportation and apoptosis-related categories, up-regulation of apoptosis-related pathways, and down-regulation of the "extracellular matrix receptor interaction" pathway, which may influence foot locomotion physiology, leading to a decrease in byssus production. This study provides mechanistic insight into the effects of OA on pearl oyster byssus, which should broaden our overall-understanding of the impacts of OA on marine ecosystem.