Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress responses and developmental stages
Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress responses and developmental stages
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DOI：
10.1046/j.1365-3040.2003.01108.x
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发表时间：
2003-11
影响因子：
7.3
通讯作者：
Kaoru Urano;Y. Yoshiba;T. Nanjo;Y. Igarashi;M. Seki;F. Sekiguchi;K. Yamaguchi-Shinozaki;K. Shinozaki
中科院分区：
文献类型：
--
作者：
Kaoru Urano;Y. Yoshiba;T. Nanjo;Y. Igarashi;M. Seki;F. Sekiguchi;K. Yamaguchi-Shinozaki;K. Shinozaki
为了表征参与多胺（PAs）生物合成的酶的基因，研究了它们的表达谱，并定量了拟南芥中PAs的水平。在拟南芥基因组中，八个基因参与多胺的生物合成进行了鉴定，这些基因的表达谱进行了分析，不仅在非生物胁迫下，以确定它们是否是胁迫诱导，组成型，或胁迫抑制，而且在各种器官，以显示其组织特异性。AtADC2和AtSPMS基因，编码精氨酸脱羧酶和精胺合成酶，明显增加响应NaCl和脱水和脱落酸处理。胁迫诱导的AtADC 2 mRNA积累与腐胺（Put）积累下NaCl和脱水处理。在寒冷条件下，AtSAMDC 2 mRNA显著增加。AtADC 2和AtSAMDC 2 mRNA在花、芽和未成熟角果等性器官中表达。PA也在性器官中积累。这些结果表明，参与PA生物合成的8个基因的转录本不仅在响应环境胁迫，而且在植物发育过程中表现出不同的表达谱。
To characterize the genes for enzymes involved in the biosynthesis of polyamines (PAs), their expression profiles were investigated and the levels of PAs in Arabidopsis thaliana quantified. In the Arabidopsis genome, eight genes involved in PAs biosynthesis were identified and the expression profiles of these genes were analysed, not only under abiotic stress to determine whether they were stress-inducible, constitutive, or stress-repressible, but also in various organs to show their tissue specificity. AtADC2 and AtSPMS mRNAs, encoding arginine decarboxylase and spermine synthase, clearly increased in response to NaCl and dehydration and abscisic acid treatments. Stress-inducible accumulation of AtADC2 mRNA correlated with putrescine (Put) accumulation under NaCl and dehydration treatments. In a cold condition, AtSAMDC2 mRNA increased significantly. AtADC2 and AtSAMDC2 mRNA were expressed in sexual organs such as flowers, buds and immature siliques. PAs also accumulated in sexual organs. These results suggest that the transcripts of eight genes involved in PA biosynthesis show different profiles of expression not only in response to environmental stress but also during plant development.