Co-expression of yeast amber suppressor tRNATyr and tyrosyl-tRNA synthetase in Escherichia coli:: Possibility to expand the genetic code
Co-expression of yeast amber suppressor tRNATyr and tyrosyl-tRNA synthetase in Escherichia coli:: Possibility to expand the genetic code
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DOI：
10.1093/oxfordjournals.jbchem.a022221
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发表时间：
1998-12-01
影响因子：
2.7
通讯作者：
Nishikawa, K
中科院分区：
文献类型：
--
作者：
Ohno, S;Yokogawa, T;Nishikawa, K
建立了一个高效的酵母tRNA（Tyr）/酪氨酰-tRNA合成酶（TyrRS）共表达系统。用几套E. coli和A.噬菌体突变体表明，表达的酵母抑制tRNA（Tyr）仅被其同源酵母TyrRS氨酰化，而不被E. coliTyrRS或其它氨酰-tRNA合成酶。这种额外的tRNA/TyrRS对有望成为开发将非天然氨基酸定点掺入蛋白质的体内系统的关键桥头。
An efficient system was developed for the co-expression of a yeast tRNA(Tyr)/tyrosyl-tRNA synthetase (TyrRS) pair in Escherichia coli. Analysis of suppression patterns using several sets of E. coli and A. phage mutants indicated that the expressed yeast suppressor tRNA(Tyr) was aminoacylated only with tyrosine by its cognate yeast TyrRS and not by E. coli TyrRS or other aminoacyl-tRNA synthetases. This extra tRNA/TyrRS pair is expected to be a key bridgehead for developing an in vivo system for the site-directed incorporation of unnatural amino acids into proteins.