From pathways to networks: connecting dots by establishing protein-protein interaction networks in signaling pathways using affinity purification and mass spectrometry.
From pathways to networks: connecting dots by establishing protein-protein interaction networks in signaling pathways using affinity purification and mass spectrometry.
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DOI：
10.1002/pmic.201400147
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发表时间：
2015-01
期刊：
影响因子：
3.4
通讯作者：
Chen, Junjie
中科院分区：
文献类型：
--
作者：
Li, Xu;Wang, Wenqi;Chen, Junjie
关键词：
信号转导是所有生物体生物活动的基础。研究信号通路，特别是在生理条件下，已成为现代生物学研究最重要的方面之一。在过去的十年中，质谱技术已广泛应用于生物学研究，并被证明可以有效解决重要的生物学问题。在这里，我们回顾了使用质谱方法理解信号网络的当前进展。我们将重点研究使用亲和纯化和质谱方法的蛋白质-蛋白质相互作用。我们讨论了亲和纯化、数据处理、功能验证和瞬时相互作用识别的障碍，并为路径特异性蛋白质组学分析提供了潜在的解决方案，我们希望有一天能够全面了解人类信号网络。
Signal transductions are the basis of biological activities in all living organisms. Studying the signaling pathways, especially under physiological conditions, has become one of the most important facets of modern biological research. During the last decade, mass spectrometry has been used extensively in biological research and is proven to be effective in addressing important biological questions. Here, we review the current progress in the understanding of signaling networks using mass spectrometry approaches. We will focus on studies of protein-protein interactions that use affinity purification followed by mass spectrometry approach. We discuss obstacles to affinity purification, data processing, functional validation, and identification of transient interactions and provide potential solutions for pathway-specific proteomics analysis, which we hope one day will lead to a comprehensive understanding of signaling networks in humans.
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