Label-free dendrimer-like silica nanohybrids for traceable and controlled gene delivery.
Label-free dendrimer-like silica nanohybrids for traceable and controlled gene delivery.
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DOI：
10.1016/j.biomaterials.2014.03.051
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发表时间：
2014-07
期刊：
影响因子：
14
通讯作者：
Xin Du;B. Shi;Youhong Tang;S. Dai;S. Qiao
中科院分区：
文献类型：
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作者：
Xin Du;B. Shi;Youhong Tang;S. Dai;S. Qiao
为了创造先进的功能性纳米载体以实现优异的基因递送性能，无荧光标记的杂化树状大分子二氧化硅纳米载体利用内体pH和细胞质谷胱甘肽（GSH）响应性自发荧光，将无毒的低分子量支化聚乙烯亚胺（PEI）连接到具有分级孔的氨基官能化树状大分子样二氧化硅纳米颗粒上，（HPSNs-NH2）。这种杂化纳米载体的特殊微结构使其不仅表现出低细胞毒性和高基因负载能力，而且表现出高基因转染效率。由GSH引起的二硫键的切割促进质粒DNA（pDNA）的释放。此外，可以使用HPSNs-AC-PEI的自发荧光实时跟踪pH和GSH控制的基因递送概况。
To create advanced functional nanocarriers for achieving excellent gene delivery performance, fluorescence label-free hybridized dendrimer-like silica nanocarriers (HPSNs-AC-PEI) were developed by using the endosomal pH and cytoplasmic glutathione (GSH) responsive autofluorescent acetaldehyde-modified-cystine (AC) to link non-toxic low molecular weight branched polyethyleneimine (PEI) onto amino-functionalized dendrimer-like silica nanoparticles with hierarchical pores (HPSNs-NH2). The specific microstructure of this hybridized nanocarrier makes it not only show low cytotoxicity and high gene loading capability, but also display high gene transfection efficiency. The cleavage of disulfide bonds caused by GSH facilitates plasmid DNA (pDNA) release. Moreover, the pH and GSH controlled gene delivery profile can be real–time tracked using the autofluorescence of HPSNs-AC-PEI.