Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.
Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.
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DOI：
10.1016/j.plasmid.2014.11.001
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发表时间：
2015-01
期刊：
影响因子：
2.6
通讯作者：
Bermudes D
中科院分区：
文献类型：
--
作者：
Morales M;Attai H;Troy K;Bermudes D
对大肠杆菌环状质粒pColE 3-CA 38（pColE 3）进行了全序列测定，发现pColE 3与肠出血性大肠杆菌（enterohemoragic E.）coli O111。我们还发现质粒的不寻常方面包括不能被限制性内切酶完全消化和不对称Phred DNA测序质量评分，相对于与正（+）链DNA一致的大肠杆菌素和免疫蛋白，正向评分显著较低。比较A260与皮绿双链DNA（dsDNA）荧光、寡绿单链DNA（ssDNA）荧光以及吖啶橙子异染染色，发现未消化的pColE 3 DNA优先以ssDNA形式染色，并与dsDNA共存。我们还在pColE 5和pColE 9中鉴定了ssDNA，但在pColE 1中没有。产生ssDNA的大肠杆菌素质粒可能代表了滚环复制质粒的一个新的亚类，并增加了大肠杆菌素和丝状噬菌体之间已知的相似性。
We sequenced the complete 7118 bp circular plasmid pColE3-CA38 (pColE3) from Escherichia coli, located the previously identified colicin components together with 2 new ORFs that have homology to mobilization and transfer proteins, and found that pColE3 is highly similar to a plasmid present in enterohemmoragic E. coli O111. We also found unusual aspects of the plasmid include the inability to be completely digested with restriction endonucleases and asymmetric Phred DNA sequencing quality scores, with significantly lower scores in the forward direction relative to the colicin and immunity proteins consistent with plus (+) strand DNA. Comparing the A260 with picogreen double-stranded DNA (dsDNA) fluorescence and oligreen single-stranded DNA (ssDNA) fluorescence as well as metachromatic staining by acridine orange, we found that the undigested pColE3 DNA stains preferentially as ssDNA and that it coexists with dsDNA. We also identified ssDNA in pColE5 and pColE9 but not in pColE1. Colicin plasmids producing ssDNA may represent a new subclass of rolling-circle replication plasmids and adds to the known similarities between colicins and filamentous phage.
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影响因子：
2.3
作者：
Lancaster, Lorna E.;Wintermeyer, Wolfgang;Rodnina, Marina V.
通讯作者：
Rodnina, Marina V.
DOI：
10.1007/bf01964380
发表时间：
1979-01-01
期刊：
EXPERIENTIA
影响因子：
--
作者：
FUSKA, J;FUSKOVA, A;MACH, J
通讯作者：
MACH, J
DOI：
10.1007/bf00334689
发表时间：
1988-05-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HORII, T;ITOH, T
通讯作者：
ITOH, T
DOI：
10.1073/pnas.74.11.4835
发表时间：
1977-01-01
影响因子：
11.1
作者：
MCMASTER, GK;CARMICHAEL, GG
通讯作者：
CARMICHAEL, GG
影响因子：
3.2
作者：
Aoki, Kazuteru;Shinohara, Miki;Itoh, Tateo
通讯作者：
Itoh, Tateo