The Structure of Native Influenza Virion Ribonucleoproteins
The Structure of Native Influenza Virion Ribonucleoproteins
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DOI：
10.1126/science.1228172
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发表时间：
2012-12-21
期刊：
影响因子：
56.9
通讯作者：
Martin-Benito, Jaime
中科院分区：
文献类型：
--
作者：
Arranz, Rocio;Coloma, Rocio;Martin-Benito, Jaime
流感病毒每年造成呼吸道疾病流行，偶尔造成大流行，构成一个重大的公共卫生问题。片段负链rna与聚合酶复合物和核蛋白（NP）相关，形成核糖核蛋白（RNPs），负责病毒转录和复制。我们描述了源自病毒粒子的天然RNPs的结构。它们呈双螺旋结构，其中两个极性相反的NP链沿着螺旋相互关联。两条链在颗粒的一端由一个短环连接，并与另一端的聚合酶复合物相互作用。这一结构将有助于揭示亲本病毒RNPs的核输入、转录和复制以及子代RNPs被病毒粒子封装的机制。
The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The segmented negative-stranded RNAs are associated with the polymerase complex and nucleoprotein (NP), forming ribonucleoproteins (RNPs), which are responsible for virus transcription and replication. We describe the structure of native RNPs derived from virions. They show a double-helical conformation in which two NP strands of opposite polarity are associated with each other along the helix. Both strands are connected by a short loop at one end of the particle and interact with the polymerase complex at the other end. This structure will be relevant for unraveling the mechanisms of nuclear import of parental virus RNPs, their transcription and replication, and the encapsidation of progeny RNPs into virions.