Comparative genome-wide segregation analysis and map construction using a reciprocal cross design to facilitate citrus germplasm utilization
Comparative genome-wide segregation analysis and map construction using a reciprocal cross design to facilitate citrus germplasm utilization
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DOI：
10.1007/s11032-009-9363-y
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发表时间：
2010-04-01
影响因子：
3.1
通讯作者：
Asins, M. J.
中科院分区：
文献类型：
--
作者：
Bernet, G. P.;Fernandez-Ribacoba, J.;Asins, M. J.
柑橘遗传资源丰富，但由于繁殖生物学复杂，农艺性状遗传研究较少，在育种中利用不足。本文采用正反交设计，研究了柑橘遗传连锁图谱中分离扭曲区域的基因组分布、细胞器DNA的遗传以及接穗柑橘连锁图谱之间的共线性。它们的父母分别是来自克莱门蒂娜的杂交柑橘Fortune和来自大柑橘的杂交柚子Chandler，它们的果实大小、味道和颜色都有很大不同。利用4个细胞器DNA标记对201个杂交种的细胞器DNA进行了遗传分析，构建了174个杂交种的连锁图谱。大约10%的以柚为母本的幼苗显示出与柑橘相同的细胞器标记，这表明它们在柑橘中可能是母体遗传的例外。大多数分离扭曲只影响等位基因频率，通常代表着花粉受精成功率的差异，这可能是由于配子因素的存在影响配子的功能和花粉-雌蕊的相互作用。将大柑橘和克莱门特柑橘的连锁图谱与之前报道的砧木种(柑橘和三叶柑橘)的连锁图谱进行比较，发现它们的共线性范围很大，这将有助于推断直源基因和QTL在柑橘物种中的位置，并有助于对柑橘种质资源进行更有用的遗传特征描述。
Citrus genetic resources are rich but underutilized in breeding because their complex reproductive biology and the scarceness of inheritance studies on agronomic traits. Here, we investigated the genomic distribution of segregation distortion regions, the inheritance of organelle DNA and colinearity between scion citrus linkage maps by using a reciprocal cross design. The parents were Fortune, a hybrid mandarin from C. clementina, and Chandler, a hybrid pummelo from C. grandis that largely differ in fruit size, taste and colour. The inheritance of organelle DNA was studied in 201 hybrids by using four organelle DNA markers, and the linkage maps were based on 174 of those hybrids. Around ten percent of the seedlings derived from the pummelo as female parent showed the same organelle markers as those of the mandarin, indicating a possible exception to their expected maternal inheritance in citrus. Most segregation distortion affects just the allele frequencies, generally representing differences in pollen fertilization success, as a likely consequence of the presence of gametal factors affecting the functionality of gametes and pollen-pistil interactions. The large extension of colinearity found when comparing the C. grandis and C. clementina linkage maps to those previously reported for rootstock species (C. aurantium and P. trifoliata), will be helpful to infer the position of orthologous genes and QTLs in citrus species and for a more useful genetic characterization of citrus germplasm collections.