The fire blight resistance QTL of Malus fusca (Mfu10) is affected but not broken down by the highly virulent Canadian Erwinia amylovora strain E2002A
The fire blight resistance QTL of Malus fusca (Mfu10) is affected but not broken down by the highly virulent Canadian Erwinia amylovora strain E2002A
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DOI：
10.1007/s10658-014-0565-8
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发表时间：
2015-03-01
影响因子：
1.8
通讯作者：
Peil, Andreas
中科院分区：
文献类型：
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作者：
Emeriewen, Ofere Francis;Richter, Klaus;Peil, Andreas
最近在海棠10号连锁群上发现了一个抗火疫病的主效QTL Mfu10，它可以解释人工接种淀粉欧文氏菌Ea222_JKI的群体中约66%的表型变异。与此同时，已有报道表明苹果火疫病QTL(MR5)的抗性可以解释80%的表型变异是菌株特有的，并被来自加拿大的高毒力菌株Ea3049(E2002a)完全克服。与Mr 5不同，Mfu10的Fusca供体只会受到这种高毒力菌株的轻微影响。在这一简短的交流中，我们证明了这种特殊的菌株不能克服青霉的抗性，而是影响Mfu10。用抗病苹果品种Idared与感病苹果品种Idared杂交产生的相同的F1后代，用Ea3049进行表型鉴定。Kruskal-Wallis分析表明，LG10上的标记与抗性水平显著相关，其中FR481a和FRM4标记的K值最高，分别为37.1和36.7。利用MAP-QTLA(R)进行区间定位和多QTL定位，结果表明Mfu10在LG10上的同一位置仍能检测到Mfu10，但只能解释41.2%的表型变异。讨论了这些结果的意义。
Recently, Mfu10 - the major QTL for resistance to fire blight was reported on linkage group 10 of Malus fusca which could explain about 66 % of the phenotypic variation in a population artificially inoculated with Erwinia amylovora strain Ea222_JKI. In the meantime, it had been reported that the resistance of the fire blight QTL of Malus x robusta 5 (Mr5), which could explain 80 % of phenotypic variation is strain specific and is completely overcome by the highly virulent strain Ea3049 originating from Canada (E2002A). In contrast to Mr5, the M. fusca donor of Mfu10 is only very slightly affected by this highly virulent strain. In this short communication, we prove that this particular strain is not able to overcome the resistance of M. fusca but affects Mfu10. The same F1 progenies, derived from a cross between the resistant M. fusca and the susceptible apple cultivar, Idared, used to detect Mfu10, were used for phenotyping with Ea3049. Although the mean shoot necrosis of all progenies was 62.4 %, marker-phenotype association determined by Kruskal-Wallis analysis showed that markers on LG10 correlate significantly to resistance levels with SSR markers FR481A and FRM4 having the highest K values of 37.1 and 36.7, respectively. Interval mapping and multiple QTL mapping (MQM) performed with MAP-QTLA (R), showed that Mfu10 could still be detected on the same position on LG10, but only explaining 41.2 % of phenotypic variation. The implications of these results are discussed.