Histamine depolarizes cholinergic interneurones in the rat striatum via a H1-receptor mediated action
Histamine depolarizes cholinergic interneurones in the rat striatum via a H1-receptor mediated action
复制标题
DOI：
10.1038/sj.bjp.0703692
复制
发表时间：
2000-11-01
影响因子：
7.3
通讯作者：
Lee, K
中科院分区：
文献类型：
--
作者：
Bell, MI;Richardson, PJ;Lee, K
1体外全细胞膜片钳记录大鼠纹状体胆碱能神经元。组胺(EC_(50)6.3um)可通过-60 mV的内向电流使这些神经元快速可逆地去极化。2组胺的作用可被H-1受体激动剂2-噻唑乙胺(50 Um)模拟，并被H-1受体拮抗剂曲普利定(1um)选择性地抑制。3电压钳条件下的离子替代实验表明，组胺激活电流由两部分组成。其中一种成分对细胞外Na+浓度敏感，另一种成分被细胞内Cs+或细胞外Ba2+4抑制。原位杂交实验表明，大鼠纹状体胆碱能神经元多数表达组胺H-1受体，少数神经元表达H-2受体。这些发现得到了单细胞RT-PCR.5的证实。结论：组胺通过H-1受体介导的机制去极化大鼠纹状体内的胆碱能神经元。
1 Whole-cell patch clamp recordings were made from rat striatal cholinergic interneurones in slices of blain tissue in vitro. Bath application of histamine (EC50 6.3 muM) was found to rapidly and reversibly depolarize those neurones through the induction of an inward current at -60 mV.2 The effects of histamine were mimicked by the H-1 receptor agonist 2-thiazolylethylamine (50 muM) and selectively inhibited by pre-incubation with the H-1 receptor antagonist triprolidine (1 muM).3 Ion substitution experiments under voltage clamp conditions revealed that the histamine activated current was comprised of two components. One component was sensitive to the concentration of extracellular Na+, whilst the other component was inhibited by intracellular Cs+ or extracellular Ba2+4 In situ hybridization experiments revealed that the majority of cholinergic interneurones in the rat striatum express the histamine H-1 receptor but few neurones express H-2 receptors. These findings were confirmed using single cell RT-PCR.5 It is concluded that histamine depolarizes cholinergic interneurones in the rat striatum via a H-1-receptor mediated mechanism.