Is it really penetration? Part 2. Locomotion of Enterococcus faecalis cells within dentinal tubules of bovine teeth
Is it really penetration? Part 2. Locomotion of Enterococcus faecalis cells within dentinal tubules of bovine teeth
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DOI：
10.1007/s00784-019-02865-5
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发表时间：
2019-12-01
影响因子：
3.4
通讯作者：
Weber, Marie-Theres
中科院分区：
文献类型：
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作者：
Kirsch, Jasmin;Basche, Sabine;Weber, Marie-Theres
目的探讨灭活粪肠球菌（Enterococcus faecalis，E。faecalis）细胞可以迁移到牙本质小管中，并且如果该过程以时间依赖的方式发生。用3%NaOCl溶液扩大根管（80号，锥度.02）后的粪菌菌株。孵育时间为7、14、21、28、35和42天。通过扫描电子显微镜（SEM）和DAPI（4，6-二脒基-2-苯基吲哚）染色处理样品用于分析。用Axio Vision程序（Zeiss，Jena，德国）的测量工具计算穿透深度。通过Kruskal-Wallis（alpha = 0.05）和Mann-Whitney U检验（p < 0.05）进行统计分析。粪菌菌株能够迁移到牙本质小管中。灭活大肠杆菌的总数和穿透深度。faecalis细胞比E.粪便。结果表明，在活体E.粪便控制，特别是在失活控制。结论失活E.由于可能的电动和渗透过程，粪细胞仍然能够迁移到牙本质小管中。因此，增加的暴露时间导致依赖于时间的穿透特性。
Objective The aim of the present vitro study was to examine the question whether devitalized Enterococcus faecalis (E. faecalis) cells can migrate into dentinal tubules and if that process takes place in a time-dependent manner.Design Sixty bovine root canals were incubated with devitalized and vital streptomycin-resistant E. faecalis strains after root canal enlargement (size 80, taper .02) with 3% NaOCl solution. Incubation times 7, 14, 21, 28, 35, and 42 days. Samples were processed for analysis by scanning electron microscopy (SEM) and DAPI (4,6-diamidino-2-phenylindole) staining. The penetration depth was calculated with the measurement tool of the Axio Vision program (Zeiss, Jena, Germany). Statistical analysis was performed by Kruskal-Wallis (alpha = 0.05) and Mann-Whitney U test (p < 0.05).Results Devitalized E. faecalis strains were able to migrate into dentinal tubules. The total number and penetration depth of devitalized E. faecalis cells was lower compared to the vital suspension of E. faecalis. It was noted, that bacterial penetration was not common to all of the dentinal tubules in the vital E. faecalis control and especially in the devitalized control. The migration took place in a time-dependent migration characteristic.Conclusions Devitalized E. faecalis cells are still able to migrate into the dentinal tubules due to possible electrokinetic and osmotic processes. Thereby, increased exposure times lead to a time-dependent penetration characteristic.