Effect of triclosan on interferon-γ production and major histocompatibility complex class II expression in human gingival fibroblasts
Effect of triclosan on interferon-γ production and major histocompatibility complex class II expression in human gingival fibroblasts
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DOI：
10.1034/j.1600-051x.2000.027010733.x
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发表时间：
2000-10-01
影响因子：
6.7
通讯作者：
Modéer, T
中科院分区：
文献类型：
--
作者：
Mustafa, M;Bakhiet, M;Modéer, T
背景、目的：研究三氯生(2，4，4‘-三氯-2’-羟基二苯醚)对4个人牙龈成纤维细胞产生干扰素-γ及主要组织相容性复合体(MHC)Ⅱ类抗原表达的影响。人牙周成纤维细胞在500和1000pg/mlrIFN-γ刺激下，可高表达MHC-II类分子。用三氯生(0.5微克/毫升)处理细胞，可减少人牙龈成纤维细胞中干扰素-γ的产生和MHC-II类的表达。抗炎剂地塞米松(1MU M)对干扰素-γ的产生和MHC-II类分子的表达也有相似的抑制作用。结论：本研究进一步支持了该抗炎剂除具有抗菌作用外还具有抗炎作用的观点。
Background, aims: The effect of triclosan (2,4,4'-trichloro-2'-hydroxyl-diphenyl ether) on the production of interferon-gamma (IFN-gamma) and the expression of major histocompatibility complex (MHC) class II antigen was studied in human gingival fibroblasts isolated from 4 individuals.Methods/Results: AII cell lines demonstrated high IFN-gamma production in 24-h cultures of human gingival fibroblasts stimulated by phytohemagglutinin (PHA) (5 mu g/ml). Human gingival fibroblasts showed a high expression of MHC class II when stimulated with 500 and 1000 pg/ml rIFN-gamma in ri-day cultures. Treatment of the cells with triclosan (0.5 mu g/ml) reduced both IFN-gamma production and MHC class II expression in human gingival fibroblast cultures. Similar inhibitory effects on IFN-gamma production and MHC class II expression were observed when the anti-inflammatory agent dexamethazone (1 mu M) was used.Conclusion: The present study further supports the view that the agent has an anti-inflammatory effect in addition to its antibacterial capacity.