Preliminary x-ray diffraction studies of EcoRI restriction endonuclease-DNA complex.
Preliminary x-ray diffraction studies of EcoRI restriction endonuclease-DNA complex.
复制标题
EcoRI 限制性内切酶-DNA 复合物的初步 X 射线衍射研究。
DOI：
10.1016/0022-2836(81)90548-9
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发表时间：
1981
影响因子：
5.6
通讯作者：
Jay,E
中科院分区：
文献类型：
--
作者：
Young,T;Kim,S;Modrich,P;Beth,A;Jay,E
EcoRI限制性内切酶与同源DNA片段5′-GAATTC CTTAAG-5′之间的复合物已结晶。空间群为P4212，a= B= 183.2A <$，c= 49.7A <$，α= β= γ= 90°。单位细胞含有四个酶单体和两个双链DNA片段。还获得了单独的酶的高质量晶体。
A complex between EcoRI restriction endonuclease and cognate DNA fragment, 5′-GAATTC CTTAAG-5′, has been crystallized. The space group is P42 1 2 with a= b= 183.2 A ̊, c= 49.7 A ̊, α= β= γ= 90°. The unit cell contains four enzyme monomers plus two duplex DNA fragments in an asymmetric unit. High quality crystals of the enzyme alone have also been obtained.