Cloning, expression analysis and sequence diversity of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI)
Cloning, expression analysis and sequence diversity of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI)
复制标题
一品红分枝诱导植原体（PoiBI）中两种不同免疫显性膜蛋白编码基因的克隆、表达分析和序列多样性
DOI：
10.1111/j.1574-6968.2011.02384.x
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发表时间：
2011
影响因子：
2.1
通讯作者：
Namba S.
中科院分区：
文献类型：
--
作者：
Neriya Y.;Sugawara K.;Maejima K.;Hashimoto M.;Komatsu K.;Minato N.;Miura C.;Kakizawa S.;Yamaji Y.;Oshima K.;Namba S.
一品红分枝诱导植原体 (PoiBI) 是一种感染一品红的植物病原细菌，与许多商业种植的一品红的自由分枝形态类型（以许多腋生芽和花为特征）有关。植原体的主要膜蛋白分为三大类，即免疫显性膜蛋白(Imp)、免疫显性膜蛋白A(IdpA)和抗原性膜蛋白(Amp)。这些膜蛋白通常用作生产植原体检测抗体的靶标。在此，我们对来自 26 个商业一品红品种的 PoiBI 菌株的 impandidpA 基因进行了克隆和测序。尽管编码的 IdpA 蛋白的氨基酸序列是不变的，但编码的 Imp 的氨基酸序列在 PoiBI 分离株之间有所不同，没有同义核苷酸取代。蛋白质印迹和免疫组织化学分析表明，Imp 的表达量超过了 IdpA，这与相关植原体疾病、western X 疾病的情况相反，后者的主要膜蛋白似乎是 IdpA，而不是 Imp。这些结果表明，即使是系统发育上相近的植原体也表达不同类型的主要膜蛋白。
Poinsettia branch-inducing phytoplasma (PoiBI) is a phytopathogenic bacterium that infects poinsettia, and is associated with the free-branching morphotype (characterized by many axillary shoots and flowers) of many commercially grown poinsettias. The major membrane proteins of phytoplasmas are classified into three general types, that is, immunodominant membrane protein (Imp), immunodominant membrane protein A (IdpA), and antigenic membrane protein (Amp). These membrane proteins are often used as targets for the production of antibodies used in phytoplasma detection. Herein, we cloned and sequenced theimpandidpAgenes of PoiBI strains from 26 commercial poinsettia cultivars. Although the amino acid sequences of the encoded IdpA proteins were invariant, those of the encoded Imp varied among the PoiBI isolates, with no synonymous nucleotide substitution. Western blotting and immunohistochemical analyses revealed that the amount of Imp expressed exceeded that of IdpA, in contrast to the case of a related phytoplasma-disease, western X-disease, for which the major membrane protein appears to be IdpA, not Imp. These results suggest that even phylogenetically close phytoplasmas express different types of major membrane proteins.