MUSCLE-FIBER TYPE COMPOSITION OF THE RAT HINDLIMB
MUSCLE-FIBER TYPE COMPOSITION OF THE RAT HINDLIMB
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DOI：
10.1002/aja.1001710303
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发表时间：
1984-01-01
影响因子：
--
通讯作者：
PHELPS, RO
中科院分区：
文献类型：
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作者：
ARMSTRONG, RB;PHELPS, RO
估计了雄性 Sprague-Dawley 大鼠 (R.norvegicus) 整个后肢中 3 种骨骼肌纤维的绝对和相对质量。对于6只大鼠，记录其总体质量，并从1只后肢的解剖中获取以下重量：32个单独的主要肌肉或肌肉部分、剩余的骨骼肌肉组织（小臀部肌肉和内在足部肌肉）、骨骼、腹股沟脂肪垫和皮肤。将来自 32 块肌肉或肌肉部分（占后肢骨骼肌质量的 98%）的纤维从组织化学上分类为快肌氧化糖酵解 (FOG)、快肌糖酵解 (FG) 或慢肌氧化糖酵解 (SO)，并确定它们的数量。使用数字化仪测量同一肌肉的纤维横截面积。然后根据纤维类型数量、纤维类型面积和肌肉质量数据计算肌肉内和整个后肢中每种纤维类型的质量。骨骼肌占后肢总质量的 71%。其中，FG 光纤占 76%，FOG 光纤占 19%，SO 光纤占 5%。因此，就肌肉质量而言，FG 纤维类型显然是大鼠后肢的主要纤维类型。将纤维型质量数据与先前研究中获取的肌肉的生理（血流量）和生化（琥珀酸脱氢酶活性）数据进行比较。这些功能特性与各种纤维类型组成的肌肉质量的比例密切相关。
The absolute and relative masses of the 3 types of skeletal muscle fibers in the total hindlimb of the male Sprague-Dawley rat (R. norvegicus) were estimated. For 6 rats, total body mass was recorded and the following weights taken from dissection of 1 hindlimb: 32 individual major muscles or muscle parts, remaining skeletal musculature (small hip muscles and intrinsic foot muscles), bone, inguinal fat pad and skin. The fibers from the 32 muscles or muscle parts (which constituted 98% of the hindlimb skeletal muscle mass) were classified from histochemistry as fast-twitch oxidative glycolytic (FOG), fast-twitch glycolytic (FG), or slow-twitch oxidative (SO), and their populations were determined. Fiber cross-sectional areas from the same muscles were measured with a digitizer. Mass of each of the fiber types within muscles and in the total hindlimb was then calculated from fiber-type population, fiber-type area and muscle-mass data. Skeletal muscle made up 71% of the total hindlimb mass. Of this, 76% was occupied by FG fibers, 19% by FOG fibers and 5% by SO fibers. Thus, the FG fiber type is clearly the predominant fiber type in the rat hindlimb in terms of muscle mass. Fiber-type mass data are compared with physiological (blood flow) and biochemical (succinate dehydrogenase activities) data for the muscles taken from previous studies. These functional properties are closely related to the proportions of muscle mass composed of the various fiber types.