Lysosome-damage-induced scattering changes coincide with release of cytochrome c
Lysosome-damage-induced scattering changes coincide with release of cytochrome c
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DOI：
10.1364/ol.32.002517
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发表时间：
2007-09-01
期刊：
影响因子：
3.6
通讯作者：
Foster, Thomas H.
中科院分区：
文献类型：
--
作者：
Wilson, Jeremy D.;Giesselman, Benjamin R.;Foster, Thomas H.
在可见波长下进行的光散射测量能够量化亚细胞形态。细胞凋亡或程序性细胞死亡与不同的形态学特征相关，例如线粒体肿胀和核浓缩以及特征性生化信号传导途径，其中许多是由细胞色素 c 从线粒体释放到细胞质中引发的。在这封信中，我们研究了光散射报告的线粒体形态变化的时间过程，以及免疫荧光显微镜测量的细胞色素 c 的亚细胞位置，以响应溶酶体光动力损伤诱导的细胞内细胞死亡信号。我们报告说，在这个系统中，细胞色素c从线粒体释放到细胞质中与线粒体形态诱导的光散射变化几乎同时发生，这进一步证明光散射有可能在未来的细胞死亡生物学研究中发挥重要作用。 (c) 2007 年美国光学学会。
Light scattering measurements made at visible wavelengths have the ability to quantify subcellular morphology. Apoptosis, or programmed cell death, is associated with distinct morphological signatures such as mitochondrial swelling and nuclear condensation as well as characteristic biochemical signaling pathways, many of which are initiated by the release of cytochrome c from the mitochondria into the cytosol. In this Letter, we examine the time course of mitochondrial morphology changes as reported by light scattering and the subcellular location of cytochrome c measured by immunofluorescence microscopy in response to intracellular cell death signaling induced by photodynamic damage to lysosomes. We report that within this system, release of cytochrome c from the mitochondria into the cytosol occurs approximately simultaneously with mitochondrial-morphology-induced light scattering changes, providing further evidence that light scattering has the potential to play an important role in future studies of cell death biology. (c) 2007 Optical Society of America.