IMMUNOHISTOCHEMICAL LOCALIZATION OF METABOTROPIC GLUTAMATE RECEPTORS, MGLUR2 AND MGLUR3, IN RAT CEREBELLAR CORTEX
IMMUNOHISTOCHEMICAL LOCALIZATION OF METABOTROPIC GLUTAMATE RECEPTORS, MGLUR2 AND MGLUR3, IN RAT CEREBELLAR CORTEX
复制标题
DOI：
10.1016/0896-6273(94)90459-6
复制
发表时间：
1994-07-01
期刊：
影响因子：
16.2
通讯作者：
MIZUNO, N
中科院分区：
文献类型：
--
作者：
OHISHI, H;OGAWAMEGURO, R;MIZUNO, N
用免疫组织化学方法观察了代谢型谷氨酸受体mGluR2和mGluR3在大鼠小脑皮质内的分布。针对含有mGluR2 C-末端部分的融合蛋白产生了抗体。免疫印迹实验表明，该抗体可与大鼠脑内mGluR2和mGluR3结合。MGluR2/3免疫反应阳性表达于高尔基细胞的胞体、树突和轴突终末，以及可能的神经胶质突起。含mGluR2/3免疫反应的高尔基体轴突终末常见于谷氨酸能杂乱纤维终末附近。结果提示，谷氨酸递质不仅可能通过树突状mGluR2/3对高尔基细胞产生调控作用，而且可能通过轴突mGluR2/3对高尔基细胞产生调控作用。
The distribution of the metabotropic glutamate receptors mGluR2 and mGluR3 was immunohistochemically examined in the rat cerebellar cortex at both light and electron microscope levels. An antibody was raised against a fusion protein containing a C-terminal portion of mGluR2. On immunoblot, the antibody reacted with both mGluR2 and mGluR3 in rat brain. mGluR2/3 immunoreactivity was expressed in cell bodies, dendrites, and axon terminals of Golgi cells, as well as in presumed glial processes. Golgi axon terminals with mGluR2/3 immunoreactivity were often encountered in the vicinity of glutamatergic messy fiber terminals. The results suggest that transmitter glutamate may exert control influences upon Golgi cells not only through dendritic mGluR2/3, but also through axonal mGluR2/3.