Smart Plasmonic Nanozyme Enhances Combined Chemo-photothermal Cancer Therapy and Reveals Tryptophan Metabolic Apoptotic Pathway
Smart Plasmonic Nanozyme Enhances Combined Chemo-photothermal Cancer Therapy and Reveals Tryptophan Metabolic Apoptotic Pathway
复制标题
智能等离子体纳米酶增强化疗光热联合癌症治疗并揭示色氨酸代谢凋亡途径
DOI：
10.1021/acs.analchem.9b01655
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发表时间：
2019
影响因子：
7.4
通讯作者：
Jin Yongdong
中科院分区：
文献类型：
--
作者：
Qi Guohua;Zhang Ying;Wang Jiafeng;Wang D;an;Wang Bo;Li Haijuan;Jin Yongdong
肿瘤微环境的调节被认为是癌症治疗的一种智能策略，但与细胞凋亡相关的代谢途径仍然是一个巨大的挑战。因此，通过将多功能碳点修饰的银/金双金属纳米壳(CDS-Ag/Au NSS，CAANSs)纳米探针作为智能等离子体纳米酶应用于肿瘤的化学-光热联合治疗，我们在肿瘤细胞治疗中取得了高效的效果，并揭示了色氨酸(Trp)代谢的凋亡途径。除了高的光热转换效率外，CAANSS还可以作为一种智能纳米酶催化细胞内的过氧化氢生成超氧阴离子(·O2-)的细胞毒性活性氧物种(ROS)，以响应温和的酸性癌细胞微环境损伤细胞DNA。更重要的是，在化学-光热联合治疗过程中，色氨酸的代谢途径揭示了色氨酸参与了氧化应激过程，在催化纳米药物过程中，氧化应激可以被分解产生过氧化氢，并进一步形成超氧阴离子以杀死细胞。目前的工作为癌症治疗提供了一个有效的平台，并有望用于与癌症相关的分子生物学研究。
Regulation of the tumor microenvironment is considered to be an intelligent strategy for cancer therapeutics, but the related metabolic pathways of cell apoptosis still remain a great challenge. Herein, by applying multifunctional carbon dot-decorated Ag/Au bimetallic nanoshells (CDs-Ag/Au NSs, CAANSs) nanoprobes as smart plasmonic nanozymes for combined chemo-photothermal cancer therapy, we achieved a high efficiency in cancer cell therapy and revealed a tryptophan (Trp) metabolic apoptotic pathway. In addition to high photothermal conversion efficiency, the CAANSs can act as a smart nanozyme to catalyze intracellular H2O2to the cytotoxic reactive oxygen species (ROS) of superoxide anion (·O2–), in response to mild acidic cancerous cell microenvironment to damage cellular DNA. More importantly, the Trp metabolic pathway during the combined chemo-photothermal therapy has revealed that the Trp participates in an oxidative stress process, which can be decomposed to produce H2O2and further formed into superoxide anions to kill cells under the catalytic nanomedicine process. The current work provides an effective platform for cancer therapeutics and is promising for cancer-related molecular biology studies.