H2-O expression in primary dendritic cells
H2-O expression in primary dendritic cells
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DOI：
10.4049/jimmunol.176.6.3548
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发表时间：
2006-03-15
影响因子：
4.4
通讯作者：
Jensen, Peter E.
中科院分区：
文献类型：
--
作者：
Chen, Xinjian;Reed-Loisel, Lisa M.;Jensen, Peter E.
H2-O是一种非多态性的II类分子，其生物学作用仍有待确定。H2-O调节H2-M功能，并且通常认为它仅在B淋巴细胞和胸腺髓上皮细胞中表达，而在树突状细胞（DC）中不表达。在这项研究中，我们报告的鉴定H2-O表达的原代小鼠树突状细胞。类似于B细胞，H2-O与DC中的H2-M相关，并且其表达在DC亚群中以及在细胞成熟和活化期间受到差异调节。脾脏和淋巴结中的原代骨髓DC和浆细胞样DC表达MHC II类和H2-M，但不表达抑制剂H2-O。相反，次级淋巴器官中的髓样DC表达H2-M和H2-O。在CD 8 α α（+）DC中，H2-O与H2-M的比率高于CD 8 α α（-）DC。在由GM-CSF和IL-4条件骨髓培养物产生的DC中，无论细胞的成熟状态如何，均未检测到H2-O表达。给予LPS可诱导骨髓DC的体内激活，并且这种激活与H2-O表达的下调有关。来自H2-O-/-和H2-O+/+小鼠的原代脾DCs向T细胞杂交瘤呈递外源性蛋白Ag的能力相似，但在混合白细胞反应中，H2-O-/- DCs比H2-O+/+ DCs诱导更强的同种异体CD 4 T细胞应答。我们的研究结果表明，H2-O有一个更广泛的作用比以前认识到在调节银介绍。
H2-O is a nonpolymorphic class II molecule whose biological role remains to be determined. H2-O modulates H2-M function, and it has been generally believed to be expressed only in B lymphocytes and thymic medullary epithelial cells, but not in dendritic cells (DCs). In this study, we report identification of H2-O expression in primary murine DCs. Similar to B cells, H2-O is associated with H2-M in DCs, and its expression is differentially regulated in DC subsets as well as during cell maturation and activation. Primary bone marrow DCs and plasmacytoid DCs in the spleen and lymph nodes express MHC class II and H2-M, but not the inhibitor H2-O. In contrast, myeloid DCs in secondary lymphoid organs express both H2-M and H2-O. In CD8 alpha alpha(+) DCs, the ratio of H2-O to H2-M is higher than in CD8 alpha alpha(-) DCs. In DCs generated from GM-CSF- and IL-4-conditioned bone marrow cultures, H2-O expression is not detected regardless of the maturation status of the cells. Administration of LPS induces in vivo activation of myeloid DCs, and this activation is associated with down-regulation of H2-O expression. Primary splenic DCs from H2-O-/- and H2-O+/+ mice present exogenous protein Ags to T cell hybridomas similarly well, but H2-O-/- DCs induce stronger allogeneic CD4 T cell response than the H2-O+/+ DCs in mixed leukocyte reactions. Our results suggest that H2-O has a broader role than previously appreciated in regulating Ag presentation.