Interstitial Cajal-like cells (ICLC) in human resting mammary gland stroma. Transmission electron microscope (TEM) identification
Interstitial Cajal-like cells (ICLC) in human resting mammary gland stroma. Transmission electron microscope (TEM) identification
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DOI：
10.1111/j.1582-4934.2005.tb00387.x
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发表时间：
2005-10-01
影响因子：
5.3
通讯作者：
Popescu, LM
中科院分区：
文献类型：
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作者：
Gherghiceanu, M;Popescu, LM
我们先前已经通过亚甲蓝（活体）染色和c-kit免疫阳性（免疫荧光和免疫组织化学）显示了人静息乳腺间质中ICLC的存在。此外，我们报告了这些ICLC在体外（原代细胞培养）的表型特征。由于肠外ICLC的鉴定需要TEM的强制性使用，我们使用这种技术，并提供了明确的证据，ICLC存在于人静止乳腺小叶内基质。根据“铂标准”（10 TEM标准ICLC的确诊），我们发现间质细胞具有以下特征：1。位置：在小管-肺泡结构之间，在非上皮间隙中; 2.小窝：相当于细胞体积的2.5%; 3.线粒体：约占细胞体积的10%; 4.内质网：光滑或粗糙，约占细胞体积的2-3%; 5.细胞骨架：存在中间和细丝，以及微管; 6.肌球蛋白粗丝：检测不到; 7.基底层：偶见; 8.间隙连接：偶尔发现; 9.通过“基质突触”与靶点紧密接触：神经纤维、毛细血管、免疫反应细胞; 10.特征性细胞质过程：i）编号：通常为2-3; ii）长度：几十μ m; iii）厚度：直径不均匀，0.1-0.5 μ m，有扩张，但从出现点看非常薄; iv）外观：念珠状，通常线粒体位于扩张中; v）分支：二分模式; vi）Ca 2+释放单位：存在; vii）网络结构系统：重叠的细胞质过程。这仍然有待于确定哪些可能的作用，我们以前建议的ICLC（例如，促分泌和/或旁分泌、未定型祖细胞、免疫监视、细胞间信号传导等）是正常或病理条件下乳腺上皮/基质平衡所必需的。
We have previously shown the existence of ICLC in human resting mammary gland stroma by means of methylene blue (vital) staining and c-kit inummopositivity (immunofluorescence and immunohistochemistry). In addition, we reported the phenotype characteristics of these ICLC in vitro (primary cell cultures). Since the identification of ICLC outside the gut requires, at this moment, the obligatory use of TEM, we used this technique and provide unequivocal evidence for the presence of ICLC in the intralobular stroma of human resting mammary gland.According to the 'platinum standard' (10 TEM criteria for the certitude diagnosis of ICLC), we found interstitial cells with the following characteristics: 1. location: among the tubulo-alveolar structures, in the non-epithelial space; 2. caveolae: similar to 2.5% of cell volume; 3. mitochondria: similar to 10% of cell volume; 4. endoplasmic reticulum: either smooth or rough, similar to 2-3% of cell volume; 5. cytoskeleton: intermediate and thin filaments, as well as microtubules are present; 6. myosin thick filaments: undetectable; 7. basal lamina: occasionally found; 8. gap junctions: occasionally found; 9. close contacts with targets: nerve fibers, capillaries, immunoreactive cells by 'stromal synapses'; 10. characteristic cytoplasmic processes: i) number: frequently 2-3; ii) length: several tens of mu m; iii) thickness: uneven caliber, 0.1-0.5 mu m, with dilations, but very thin from the emerging point; iv) aspect: moniliform, usually with mitochondria located in dilations; v) branching: dichotomous pattern; vi) Ca2+ release units: are present; vii) network labyrinthic system: overlapping cytoplasmic processes.It remains to be established which of the possible roles that we previously suggested for ICLC (e.g. juxta- and/or paracrine secretion, uncommited progenitor cells, immunological surveillance, intercellular signaling, etc.) are essential for the epithelium/stroma equilibrium in the mammary gland under normal or pathological conditions.