Does High Load of Oxidants in Human Semen Contribute to Male Factor Infertility?
Does High Load of Oxidants in Human Semen Contribute to Male Factor Infertility?
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人类精液中氧化剂含量高是否会导致男性不育？
DOI：
10.1089/ars.2011.4461
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发表时间：
2012-04-01
影响因子：
6.6
通讯作者：
Shi, Hui-Juan
中科院分区：
文献类型：
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作者：
Chen, Hong;Zhao, Hong-Xin;Shi, Hui-Juan
活性氧（ROS）的基础生成是男性生殖功能所必需的，而高ROS水平可能与精子质量低和男性不育有关。我们研究了1092名男性因素不育（MFI）患者和50名精液特征正常的供体的整个精液中ROS水平与精子质量之间的关系。ROS水平与精子畸形率、头部畸形率、精子畸形指数呈显著正相关。此外，我们研究了高ROS水平的MFI患者的精浆是否会影响精液特征正常的供体的精子活力。将来自具有正常精液特征的供体的新鲜人精子与来自不育男性的精浆交叉培养后，在不同ROS水平下测量精子活力。MFI患者的精浆显著降低精子的活力，并且降低率随着精浆中ROS水平的增加而增加。另一方面，我们发现活性氧水平在最低第25百分位数的MFI患者与具有正常精液特征的供体具有相似的活性氧水平。总的来说，我们的观察导致的假设，氧化应激在MFI的发展中起着至关重要的作用，其中具有高ROS水平，但不是那些具有低ROS水平。抗氧化剂。氧化还原信号。16，754-759。
Basal generation of reactive oxygen species (ROS) was essential for male reproductive function, whereas high ROS levels may be linked to low quality of sperm and male infertility. We examined the associations between ROS levels in whole ejaculates and sperm quality among 1092 male factor infertility (MFI) patients and 50 donors with normal semen characteristics. ROS levels were significantly positively correlated with abnormal morphology rate, head defect, and sperm deformity index. Further, we investigated whether seminal plasma from MFI patients with high ROS levels affects sperm motility from donors with normal semen characteristics. After cross-culturing fresh human sperm from donors possessing normal semen characteristics with seminal plasma from infertitle men, sperm motility was measured at different ROS levels. Seminal plasma from MFI patients significantly reduced motility of sperm and the reduction rate increased with increasing ROS levels in seminal plasma. On the other hand, we found MFI patients with the ROS levels in the lowest 25th percentile had similar ROS levels to donors with normal semen characteristics. Collectively, our observations lead to the hypothesis that oxidative stress plays a critical role in the development of MFI among those with high ROS levels, but not those with low ROS levels. Antioxid. Redox Signal. 16, 754-759.