A novel sigma class glutathione S-transferase gene in freshwater planarian Dugesia japonica: cloning, characterization and protective effects in herbicide glyphosate stress
A novel sigma class glutathione S-transferase gene in freshwater planarian Dugesia japonica: cloning, characterization and protective effects in herbicide glyphosate stress
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淡水涡虫 Dugesia japonica 中新型 sigma 类谷胱甘肽 S-转移酶基因：克隆、表征及其对除草剂草甘膦胁迫的保护作用
DOI：
10.1007/s10646-020-02173-9
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发表时间：
2020-02
期刊：
影响因子：
2.7
通讯作者：
De-Zeng Liu
中科院分区：
文献类型：
--
作者：
He-Cai Zhang;Yu-Juan Yang;Ke-Xue Ma;Chang-Ying Shi;Guang-Wen Chen;De-Zeng Liu
草甘膦作为世界上最畅销的除草剂，在自然环境中分布广泛，对生态安全和人类健康的影响越来越受到人们的关注。谷胱甘肽S转移酶(GSTs)是一个功能齐全的同工酶超家族，具有保护细胞免受外源有毒物质伤害的功能。本研究首次采用RACE方法克隆了淡水浮游动物日本涡虫GST基因(命名为DJA-GST)的全长cDNA序列。DJA-GST全长由706个核苷酸组成，编码200个氨基酸的多肽。DJA-GST在N-末端和C-末端有两个具有代表性的GST结构域。保守的GST-N结构域包括G-位点Y8、F9、R14、W39、K43、P52和S64，而可变的GST-C结构域包括H-位点K104、V156、D159和L161。序列分析、系统发育树重建和多重比对表明，DJA-GST属于GST超家族中的Sigma类。系统研究了草甘膦胁迫下GST基因表达谱、GST酶活性和丙二醛含量的变化规律，并分析了三者之间的相关性。本工作为进一步研究DJA-GST的确切功能奠定了分子基础，对评价除草剂草甘膦的生态环境效应具有重要意义。
As the top-selling herbicide in the world, glyphosate distributes widely in natural environment and its influence on the ecological security and human health has attracted more and more concern. Glutathione S-transferases (GSTs) are a well-characterized superfamily of isoenzymes for cellular defense against exogenous toxic substances and therefore protect organisms from injury. In this study, the complete cDNA sequence of GST gene (named as Dja-GST) in freshwater planarian Dugesia japonica was firstly cloned by means of RACE method. The full-length Dja-GST comprises of 706 nucleotides which encodes a polypeptide of 200 amino acids. Dja-GST has two representative GST domains at the N- and C-termini. The conservative GST-N domain includes G-site Y8, F9, R14, W39, K43, P52 and S64, while the variable GST-C domain contains H-site K104, V156, D159 and L161. Sequence analysis, phylogenetic tree reconstruction and multiple alignment collectively indicate that Dja-GST belongs to the Sigma class of GST superfamily. Also, GST gene expression profile, GST enzymatic activity and MDA content in response to glyphosate exposure were systematically investigated and the correlations among them were analyzed. This work lays a molecular foundation for further exploring the exact functions of Dja-GST and gives an important implication for evaluating the ecological environment effects of herbicide glyphosate.
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影响因子：
5.8
作者：
Espinoza, Herbert M.;Shireman, Laura M.;McClain, Valerie;Atkins, William;Gallagher, Evan P.
通讯作者：
Gallagher, Evan P.
影响因子：
4.5
作者：
S. Koutsogiannaki;S. Franzellitti;E. Fabbri;M. Kaloyianni
通讯作者：
S. Koutsogiannaki;S. Franzellitti;E. Fabbri;M. Kaloyianni
影响因子：
4.5
作者：
He-Cai Zhang;Kexue Ma;Yu-Juan Yang;Changying Shi;Guangwen Chen;Dezeng Liu
通讯作者：
He-Cai Zhang;Kexue Ma;Yu-Juan Yang;Changying Shi;Guangwen Chen;Dezeng Liu
DOI：
10.1016/j.cca.2004.10.008
发表时间：
2004-10-01
影响因子：
3.9
作者：
Morales, AE;Pérez-Jiménez, A;Cardenete, G
通讯作者：
Cardenete, G
影响因子：
4.8
作者：
Cho, SG;Lee, YH;Choi, EJ
通讯作者：
Choi, EJ